厌氧氨氧化菌的发现改变了生物脱氮途径，使污水处理曝气量节省60%。目前厌氧氨氧化的应用研究主要集中在高氨氮废水处理，而城市污水厌氧氨氧化的研究还处于初期阶段，其瓶颈是难以实现稳定短程硝化。本项目预研试验发现，缺氧条件下游离亚硝酸（FNA）对亚硝酸盐氧化菌（NOB）的抑制远大于对氨氧化菌（AOB）的抑制，因此缺氧FNA抑菌效应有望突破上述瓶颈。 本项目将在预研基础上，首次研究缺氧FNA对AOB与NOB的抑菌效应；从能量代谢与群体效应的角度，探讨缺氧FNA对AOB与NOB的抑菌机理；确定缺氧FNA抑菌实现硝化过程亚硝酸盐积累的条件，结合缺氧FNA抑菌对系统N2O产生的影响，建立城市污水短程硝化厌氧氨氧化脱氮新工艺。并首次在中试规模试验验证结果，建立基于缺氧FNA抑菌的城市污水短程硝化厌氧氨氧化脱氮系统优化控制方法，为城市污水生物脱氮节能降耗提供新途径。

The discovery of anearobic ammonium oxidizing (Anammox) bacteria change the pathway of biological nitrogen removal, which could save 60% of the oxygen demanded for aeration. However, the research about anammox focused mainly on the N-rich wastewater treatment, and the study for sewage treatment via anammox was still in lab-sclae stage.The difficulty of achieving nitrogen removal from domestic wastewater via anammox is to accomplish stable nitritation. Our preliminary test indicated that the inhibition of free nitrous acid (FNA) on nitrite oxidation bacteria (NOB) was much more serious than ammonium oxidation bacteria (AOB) under anoxic condition, which make it possible to achiev stable nitritation in sewage treatment system. The effect of FNA inhibition on AOB and NOB will be firstly studied under anoxic condition based on our preliminary results. The mechanism of anoxic FNA inhibition on AOB and NOB will be studied in view of energy metabolism and quorum sensing. The required condition for achieving nitrite accumulation under anoxic FNA inhibitiong will be investigated. Meanwhile, the effect of anoxic FNA inhibition on nitrous oxide production will be also studied. A novel nitritation anammox process treating domestic wastewater will be developed. Above results obtained will be firstly evaluated in the pilot scale reactor. At last the optimal control strategies of nitrogen removal process via anammox based on anoxic FNA inhibition will be proposed, which will provide a new approach for sving energy consumption of domestic wastewater treatment.