基于HSV-2 gD介导的识别及膜融合探讨洁泽1号扶正解毒作用的分子机制

HSV-2病毒引起的生殖器疱疹已成为全世界高发的性传播疾病，妊娠合并HSV-2可危及新生儿生命和高发神经系统发育缺陷，严重影响出生人口质量。目前尚无疫苗和妊娠安全用药。本课题在前期洁泽1号防治HSV-2病毒感染取得良好疗效的基础上，以病毒糖蛋白gD介导的对宿主细胞黏附及膜融合机制和宿主细胞的TLR诱发天然免疫这两方面为切入点，从组织、细胞、分子等层次深入研究中药的"扶正解毒"作用及其对HSV-2致病力和宿主免疫能力的影响，以期阐明洁泽1号防治生殖器疱疹的作用机理和分子机制，为进一步指导临床用药提供理论依据。本项目将从一个侧面充实中药抗病毒作用的科学内涵,为中医药防治感染性疾病开拓新的思路并赋予其新的诠释。

Genital herpes caused by HSV-2 has been the higher risk of sexually transmitted diseases.Pregnant women with HSV-2 can endanger neonatal life and have high risk in the development of fetal nervous system defect, The infection of HSV-2 can serious influence on the quality of the population. However, there is no vaccine and pregnancy security medicine in the treatment of HSV-2. This subject based on the effects of JZ-No.1 on preventing and treating HSV-2 virus infection in earlier stage,Both of adhesion and membrane fusion mechanism of the host cell are mediated by the virus glycoprotein gD and the host cell TLRs induce natural immune as the breakthrough point, Our project is to study the molecular mechanism of JZ-No.1 (an traditional Chinese medicine decoction)on strengthening body resistance and detoxification with recognition and membranous fusion mediated by HSV-2 Glycoprotein D in tissue ,cells and molecular levels..Thus we can predict the role of traditional Chinese medical prescription preventing and treating genital herpes through strengthening Vital-qi's power to resist pathogenic factors, and can enrich new ideas and give them new interpretation in the theory basis for further guiding clinical medication.