神经母细胞瘤（neuroblastoma，NB）具有高度侵袭性和转移性，其发病机制尚不明确。申请人前期研究发现18F-FDG PET显像是预测NB预后的一种有效手段，为治疗提供指导价值。研究表明ASPM参与胚胎发育、细胞有丝分裂及肿瘤发生，尤其与肿瘤的复发及转移密切相关；预实验发现，ASPM与NB患者的预后密切相关；相对于NB低危组，高危组肿瘤标本中ASPM表达明显增高，且与肿瘤干细胞的分化相关。由此推测，ASPM在肿瘤干细胞分化及NB的发生、发展中发挥重要作用。本项目拟探讨：1.ASPM是否对NB恶性行为具有调控作用并明确其分子机制；2.明确ASPM与肿瘤干细胞分化之间的关系；3.体内动物模型验证ASPM对NB生长的调控作用，并采用18F-FDG PET显像评估ASPM沉默后的生物学效应。旨在：以ASPM为切入点，探讨NB发生发展及肿瘤干细胞分化的分子机制，为临床NB治疗提供新思路。

Neuroblastoma (NB) is highly invasive and metastatic, and its pathogenesis is still unclear. The applicant's previous study found that 18F-FDG PET imaging is an effective means to predict the prognosis of NB, providing guidance for treatment. Studies have shown that ASPM is involved in embryonic development, cell mitosis and tumorigenesis, especially related to tumor recurrence and metastasis; pre-experiment found that ASPM is closely related to the prognosis of patients with NB; compared with NB low-risk group, ASPM expression in high-risk group tumor specimens Significantly increased, and associated with the differentiation of cancer stem cells. It is speculated that ASPM plays an important role in tumor stem cell differentiation and the occurrence and development of NB. This project is to explore: 1. Whether ASPM has a regulatory effect on NB malignant behavior and clarifies its molecular mechanism; 2. Defining the relationship between ASPM and tumor stem cell differentiation; 3. In vivo animal model to verify the regulation of ASPM on NB growth, and The biological effects of ASPM silencing were evaluated by 18F-FDG PET imaging. The aim is to use ASPM as an entry point to explore the molecular mechanism of NB development and tumor stem cell differentiation, and provide new ideas for clinical NB treatment.