脾肾阳虚型溃疡性结肠炎大鼠Notch-mTOR-能量代谢轴变化及四神丸干预机制研究

脾肾阳虚证是慢性溃疡性结肠炎(UC)的主要证型，其临床辨证存在一定主观性，结合客观指标能提高辨证的准确性。作为当前的研究热点，Notch-mTOR信号与细胞能量代谢相互关联，参与了脾肾阳虚型UC发病过程，提示脾肾阳虚型UC可能存在Notch-mTOR-能量代谢信号轴的变化。而四神丸是有效治疗UC的基础方，具有良好的临床和实验基础，然其作用机制不明。我们拟采用经典的脾肾阳虚型UC动物模型，经四神丸治疗后，分别采用PCRarray分析、流式细胞术等试验方法，评价其治疗前后能量代谢水平和相关基因表达的变化，探索脾肾阳虚型UC大鼠Notch-mTor-能量代谢轴变化特征，为脾肾阳虚型UC的辨证提供客观化证据；并从Notch-mTOR-能量轴角度以方测证，明确四神丸能否通过调控Notch-mTOR信号介导能量代谢变化达到治疗目的。这对于脾肾阳虚型UC的诊断及治疗有重要临床价值和科学意义。

Spleen-Kidney-Yang deficiency syndrome is the main syndromes in UC chronicperiod. Its syndrome differentiation has certain subjectivity. Combining with objective indicators can improve the accuracy of syndromedifferentiation.Signaling pathway of Notch bonded with mTOR can regulate cell energy metabolism, is closely related with Spleen-Kidney-Yang Deficiency Ulcerative Colitis(SKYDUC) disease . It is hinded that there are Changes of Notch-mTOR-Energy Metabolic Axis.We intend to adopt the classical UC animal model of SKYDUC,and evaluate curative effect, the level of energy metabolism, gene expression of SSW treated SKYDUC.Exploring the characteristics of Notch-mTor-energy metabolism axis in SKYDUC rats can provides objective evidence for differentiation of syndrome of SKYDUC and explore the possible mehanism of SSW treated replased SKYDUC，and determine whether SSW can achieve therapeutic purpose by regulating Notch-mTOR signal to mediate energy metabolism changes. It has important clinical value and scientific significance for the diagnosis and treatment of SKYDUC.