肿瘤基因放射治疗是近年来发展起来的抗肿瘤治疗新方法。这种方法将放射与基因治疗联合应用于治疗肿瘤，而该方法面临的难题是筛选到理想的“靶基因”。最新研究报道：多聚胞嘧啶结合蛋白1（Poly(C)-binding protein 1，PCBP1）能够在多水平进行肿瘤细胞功能的精细调控，然而该基因协同辐射在肿瘤细胞DNA损伤应答和凋亡中的功能尚未见报道。本项目拟建立PCBP1的肿瘤细胞研究模型， 采用X射线和碳离子辐照癌细胞，利用微束平台、免疫荧光等技术实现PCBP1对辐射诱导的DNA双链断裂以及DNA损伤蛋白招募的动态时空分析，通过免疫共沉淀、转录组测序、荧光定量PCR、Western blot等技术研究辐照致PCBP1调控的肿瘤细胞非p53凋亡信号通路。研究旨在揭示PCBP1在肿瘤细胞辐射抗拒中的分子机理,为设计以该基因为靶点的放射治疗增敏技术，进一步提高辐射治癌疗效提供一些生物学依据。

Gene-radiation therapy is a new method for the development of anti tumor therapy in recent years. The method is the combination of radiation and gene therapy in the treatment of cancer, and the problem is to select the ideal target gene. Latest research report: as tumor suppressor genes PCBP1 (Poly (C) -binding protein 1, also known as hnRNP E1 or alpha CP-1), regulates gene expression in multiple level , such as transcription, mRNA processing, mRNA trafficking, mRNA stabilization and translation control. However the combination of PCBP1 and radiation in DNA damage response and tumor cells apoptosis is not reported. The project intends to establish a research model of PCBP1 tumor cells to study the spatial and temporal role of PCBP1 in DNA double-strand breaks and DNA damage response using X-rays and carbon ions irradiated cancer cells, and study the role of PCBP1 in p53 independent apoptosis signaling pathway using immunoprecipitation ,RNA sequencing, quantitative PCR, western blot, etc.Thus this project will study the molecular biological mechanisms of radiation resistance of cancer cells to provide a biological basis for radiation tumor gene therapy research, and promote theoretical innovation radiobiology research..