长链非编码RNA在肿瘤进展中发挥重要作用。我们前期研究鉴定出一条新的lncRNA NR-037939，其表达激活与胃癌恶性表型高度相关。体内外实验证实NR-037939促进胃癌肿瘤生长转移，但机制未知。进一步的RNA-seq及通路分析显示，NR-037939参与糖代谢途径并调控糖能量代谢关键因子SIRT1的表达。因此提出假设：NR-037939可能通过SIRT1介导的糖代谢重编程促进胃癌进展。本项目拟首先从体内外模型证实NR-037939调控胃癌细胞糖代谢重编程（促进糖酵解、抑制氧化磷酸化）的关键作用；体外过表达/沉默实验确定NR-037939调控SIRT1介导的糖代谢异常对胃癌细胞恶性表型的影响，并鉴定NR-037939调控作用的上下游分子机制。最后结合动物模型及临床样本明确NR-037939在调控糖代谢促进胃癌进程中的作用机制。该研究有望揭示胃癌相关lncRNA调控胃癌进展的新机制。

Long noncoding RNA plays an important role in tumor progression. Our previous study identified a new lncRNA, named lncRNA NR-037939, which is highly expressed in gastric cancer and significantly correlated with the malignant phenotype of gastric cancer. Moreover, we confirmed that lncRNA NR-037939 promotes the growth and metastasis of gastric cancer in vitro and in vivo. However, the detailed mechanism need to be clarified. Furthermore, RNA-seq and pathway analysis indicate that NR-037939 is involved in the pathway of glucose metabolism and regulates the expression of SIRT1, a key regulated gene for glycometabolism. Based on these interesting findings, we hypothesized that lncRNA NR-037939 may promote the progression of gastric cancer through SIRT1-mediated reprogramming of glucose metabolism. This project intends to first confirm the critical role of NR-037939 in the regulation of glucose metabolism reprogramming by promoting glycolysis and inhibiting oxidative phosphorylation of gastric cancer cells in vitro and in vivo. Using overexpression / silencing experiments, we aim to testify the effect of SIRT1 mediated glycometabolism on the regulated malignant phenotype of gastric cancer cells, and then dissect the detailed molecular mechanism to explore the upstream and downstream signaling pathway of lncRNA NR-037939. Finally, we verify the important role of lncRNA NR-037939 on the progression of gastric cancer by regulating SIRT1 mediated glycometabolism in mouse model and clinical samples of gastric cancer. This project will provide the new insight into the mechanistic regulation of this new lncRNA NR-037939 on the progression of gastric cancer.