染色质重塑复合物通过水解ATP释放的能量来改变核小体的结构，从而调节转录调控因子等对染色质的可接近性以实现对基因的表达调控。BAF复合体（由15亚基组成）中的核心复合物，包括4个亚基Brg-1/Brm、BAF47、BAF155和BAF170，发挥核心的染色质重塑功能。为了深入理解该核心复合物的装配结构机理和功能机制，我们综合运用多学科交叉手段，研究以下三个方面的内容：人源BAF复合体中BAF170-BAF47二元复合物的结构与功能研究；酵母源SWI/SNF复合体中Swi3-Snf5二元复合物的结构与功能研究；BAF复合体中核心复合物的装配过程研究。本项目的研究成果将为理解染色质重塑的分子机理产生深远影响，并揭示BAF（SWI/SNF）复合体的功能保守性及装配特异性，并为阐明由于BAF复合体功能紊乱而导致的相关疾病的发生发展上提供理论依据和前期积累。

Chromatin remodeling complexes use the energy of ATP hydrolyzation to change the structure of nucleosome, which regulating the chromatin accessibility by transcription factors and thereby achieving gene expression regulation. The core complex, including four subunits Brg-1/Brm, BAF47, BAF155 and BAF170,is the key player in the remodeling activities of the BAF complex containing 15 subunits. However, the molecular mechanism of the core complex assembly and regulation remain elusive. Here, we will use multidisciplinary methods to study three following parts: structural and functional studies of BAF170-BAF47 binary complex of human BAF complex; structural and functional studies of Swi3-Snf5 binary complex of yeast SWI/SNF complex; In vitro assembly and structural studies of the core Brg-1/Brm- BAF47-BAF155-BAF170 quaternary complex. Our results will have profound effect on the molecular mechanism of chromatin remodeling and reveal the mechanism of functional conservation and assembly specificity of the BAF complex, and will clarify the developmental process of diseases causing by dysfunction of the BAF complex.