云南宣威肺癌高发，然而其驱动基因和发病机制至今未见阐明。我们前期采用mRNA芯片、Real-time PCR和Cellomics高通量细胞增殖基因筛选系统，获得宣威肺癌特异促增殖基因PPP1R14D（结果一~二）；然后免疫组化检测，PPP1R14D在宣威肺癌高表达，并且与T分期显著相关（结果三）；在宣威肺癌XLA-07细胞，敲减PPP1R14D，肺癌增殖能力降低，出现G2/M期阻滞（结果四）。PPP1R14D 可能通过PP1/Mps1通路，调控纺锤体组装，干预有丝分裂。进一步实验发现，敲减PPP1R14D，磷酸化PP1 增多，Mps1和pMps1均减少，免疫共沉淀证实PPP1R14D与PP1存在关联（结果五），提示PP1/Mps1可能是PPP1R14D的下游通路。本项目拟阐明PPP1R14D介导PP1/MPS1，干预纺锤体组装，调控肺癌增殖的机制，为宣威肺癌的防治提供新的干预靶点。

The incidence of Xuanwei lung cancer is the highest, but so far its pathogenesis has not been elucidated. We obtained Xuanwei lung cancer-specific proliferative gene PPP1R14D (Result One ~ Two) using mRNA microarray, Real-time PCR and Cellomics high-throughput proliferation genes screening system; then detected it by immunohistochemistry, PPP1R14D is highly expressed in Xuanwei lung cancer, and significantly correlated with T stage (Result Three); in Xuanwei lung cancer cells line XLA-07, knockdown PPP1R14D, the proliferation ability of lung cancer was decreased, appeared G2/M phase arrest (Result Four). PPP1R14D may affect PP1/Mps1 signaling, then regulated spindle assembly and mitosis. Further experiments found that knockdown PPP1R14D, phosphorylation of PP1 was increased, Mps1 and pMps1 were decreased, immunoprecipitation confirmed PPP1R14D was associated with PP1 (Result Five), these results suggested that PP1/Mps1 is the downstream pathway of PPP1R14D. This project intends to clarify the mechanism on PPP1R14D affected PP1/MPS1 signaling, then intervented spindle assembly and regulated proliferation of lung cancer, which would provide a new target for the prevention and treatment of Xuanwei lung cancer.