云南道地药材滇黄精多糖结构及降血糖机理研究

糖尿病是严重危害健康的全球公共卫生问题，虽然降糖西药能有效控制高血糖，但不良反应严重，植物活性多糖在防治糖尿病及其并发症的优势已受到医药界普遍关注。滇黄精在云南彝族民间长期用于治疗糖尿病，疗效确切，但其活性物质基础和作用机制尚未明了。项目组前期研究首次发现其总多糖和从中分离得到的均一多糖PPS-I具有显著的降血糖作用，成果已获专利2项。本研究基于已有药效学和化学基础，拟用GC-MS、IR、NMR、X射线衍射等现代分析方法，研究滇黄精中降血糖活性均一多糖的理化性质，阐明其分子结构信息。并采用自发性和实验性2型糖尿病动物模型，以及离体肝细胞和脂肪细胞模型，从体内和体外探讨滇黄精多糖降血糖的作用及机理，并初步分析其结构与降血糖活性之间的关系。研究成果有望开发成具有自主知识产权的新型防治糖尿病天然药物，为后续的产品研发、变资源优势为产业优势提供强有力的科学支撑。

Diabetes is now a common and serious global health problem. Although western medicine can effectively control their glucose levels, they also have unwanted and serious side effects. Plant polysaccharides in the prevention and treatment of diabetes mellitus and its complications advantage has been widespread concerned by the pharmaceutical industry. Rhizoma Polygonatum kingianum is used for treatment of diabetes mellitus in Yunnan Yi folk for a long term, but its material basis for efficacy and mechanism of anti-diabetes remains unclear. The total polysaccharide and the polysaccharide that separated from it have remarkable effect on reducing blood sugar in the preliminary research, which has been authorized 2 patents. This study based on the pharmacodynamics and chemical basis, the specific glycosidase cutting technology with GC-MS, IR, NMR, X ray diffraction and other modern analysis method will apply to clarify the molecular structure information of polysaccharides. At the same time, the spontaneous and experimental of type 2 diabetes mellitus animal model, the model of liver cells and fat cells will be used to study the effect and mechanism of hypoglycemic activity of a polysaccharide from the Rhizome Polygonatum kingianum. After that, preliminary analyze the relationship between its structure and hypoglycemic activity. The fruits of the research will expected to be developed into new drug with independent intellectual property rights, which will provide a strong scientific support for the follow-up product development.