本项目针对内蒙古地区夏季丰富光照资源的利用，与设施环境中高温、低CO2对蔬菜栽培阻碍之间的矛盾，利用智能温室精准操控试验条件，以黄瓜为试材，通过在高温环境中加富CO2，研究气孔的生理机制和气体的交换机制。依据对叶表气孔孔径和气孔导度日变化差异和对幼芽到成熟叶表的气孔密度和分布变化差异，分析高温、加富CO2条件下温室黄瓜叶表气孔运动和发育规律；测定脱落酸和生长素含量变化，利用激光共聚焦显微镜测定叶表NO和H2O2含量差异，研究黄瓜气孔发育转录因子的基因表达随时间的变化，探讨高温、加富CO2条件下温室黄瓜叶表气孔运动和发育的调节机制；测定光合传递效率和碳同化效率的差异，探究高温、CO2加富条件下气孔行为与光合作用的关系。研究结果不仅为北方地区周年黄瓜生产提供科学的理论依据，为设施黄瓜综合配套栽培技术与管理技术提供技术支撑，而且可望对高温高浓度CO2调控植物环境适应性的机理提供新的认识。

To solve the contradiction between the use of abundant light resources and the obstacle of high temperature and low CO2 concentration in the facilities environment in summer of Inner Mongolia area, this study is focus on physiological mechanism of stomatal and mechanism of gas exchange of cucumber leaf under high temperature and CO2 enrichment. Based on the difference of diurnal variation of stomatal aperture and stomatal conductance, and the difference of stomatal density and distribution from bud to mature leaves, the law of stomatal movement and development of cucumber leaves under high temperature and CO2 enrichment will be analyzed. By measuring the content of abscisic acid and auxin, the content of NO and H2O2, and the changes of gene expression in the stomata development, the regulation mechanism of stomatal movement and development of cucumber leaves under high temperature and CO2 enrichment will be illuminated. To investigate the relationship between stomatal behavior and photosynthesis under high temperature and CO2 enrichment, the difference of photosynthetic transfer efficiency and carbon assimilation efficiency were measured. After completing the research, the study will provide scientific theoretical basis of greenhouse cucumber production in the summer of north China and the comprehensive cultivation technology and management technology of greenhouse cucumber. Furthermore, it will be expected to provide a new understanding of the adaptive mechanism of plant under high temperature and high CO2 concentration environment.