拟南芥叶表皮细胞具有相邻细胞锯齿状嵌合的形状，是研究细胞形态发生和细胞-细胞信号传递的理想模式系统。近几年的研究表明，生长素激活ROP2和ROP6信号分别调控细胞凸起和凹陷的形成。但是，参与ROP信号通路的新组分还有待于进一步挖掘。本研究通过正向遗传学方法在ROP6过量表达背景下筛选到了一个增强子Angustifolia，该基因突变能加剧ROP6过量表达的叶表皮细胞表型。我们的初步研究结果表明，Angustifolia调控叶表皮细胞中生长素运输蛋白PIN1的极性定位，并能影响ROP6活性。在此工作基础上，将进一步探索Angustifolia调控PIN1囊泡运输，以及通过筛选Angustifolia互作蛋白，明确Angustifolia参与ROP6 信号调控细胞形态发生的分子机制。该研究不仅有助于深入了解细胞形态发生的分子机制，同时对植物生长发育的细胞水平调控提供理论依据。

Arabidopsis leaf pavement cell has interlocking jigsaw-puzzle shape, which provides an ideal model system to investigate cell morphogenesis and cell-to-cell communication. It has been reported that auxin activates the Rho small GTPase ROP2 and ROP6 signals to coordinate the formation of lobes and indentations respectively. However, the new components involved in ROP signaling are still need to be uncovered. In this study, we used forward genetics method to screen enhancers in ROP6 overexpression background, and found an enhancer mutant, whose gene encoding Angustifolia. The mutation could enhance pavement cell phenotypes induced by ROP6 overexpression. Our preliminary results have shown that Angustifolia not only regulates polar localization of auxin transporter PIN1 in leaf pavement cells, but also controls ROP6 activity. Based on previous work, we will further study the connection between Angustifolia and PIN1 trafficking and identify Angustifolia-interacting proteins, which will explains the mechanism of Angustifolia involved in ROP6-dependent signaling in the regulation of leaf pavement cell morphogenesis. This study will not only make us better understand the mechanism of cell morphogenesis but also contribute to better know the mechanism of plant growth and development through cell biology level.