提高种公猪繁殖性能是畜牧科学研究的焦点之一，精子受精能力是体现公畜繁殖性能的重要指标，并与精液蛋白的作用密切相关。猪精液蛋白与精子受精能力和公猪繁殖性能的关系与作用机制亟待阐释。本项目拟利用统计学方法从大群体公猪中筛选出常规精液指标合格、但繁殖性能存在差异的两个群体（高繁组和低繁组）为主要研究对象；通过双向电泳、免疫印记、ELISA等技术对两组公猪精液中的蛋白（如OPN、CRISP1等）进行分析和定量，再联合公猪繁殖成绩建立相关精液蛋白与繁殖性能的相关性模型；然后通过检测目标蛋白在公猪生殖器官、精清和精子的表达分布，并利用体外受精、人工授精等技术研究目标蛋白在受精过程中的作用，探索并阐释目标蛋白对精子受精能力的影响及作用机制。本项目研究可为准确评定精液受精能力提供蛋白标记、提高人工授精和种公猪繁殖效率；还可为种公猪育种提供重要参考依据；同时也可为人类生殖不孕不育的研究和治疗提供参考。

The improvement of boar reproductive performance is one of the scientific focuses in animal husbandry. The sperm fertilization ability, which closely related to the roles of semen proteins, is one of the important indicators reflecting male animal reproductive ability. The relationships and mechanism between boar semen proteins and sperm fertilization ability or boar breeding performance are eagerly need to be elucidated. This project intends to use statistical methods for selecting two animal groups (high breeding and low breeding group) as the main research objects from a large boar group based on a criterion of qualified conventional semen indexes but different breeding performance. Then with two-dimensional electrophoresis, immune signatures and ELISA technologies to analyze and quantify the semen proteins (e.g., OPN, CRISP1, etc.) of the two group animals mentioned above. And then joint with boar breeding achievements, a correlation model related sperm protein and reproductive performance will be set up. On the other hand, the expression and distribution of the objective proteins were detected in boar reproductive organs, seminal plasma and sperm. And the effect of objective proteins in the process of fertilization was studied by using artificial insemination, in vitro fertilization and related technologies, so as to explore and elucidate the effect and mechanism of objective protein actions with the sperm fertilization ability. This project can provide protein markers for accurate evaluation of semen fertilization capacity, improving the efficiency of artificial insemination and boar reproduction; also it can provide important reference basis for boar breeding; At the same time, it can also provide reference for the research and treatment of infertility in human reproduction.