Pokemon 在乳腺癌侵袭转移中的作用及机制研究

乳腺癌是女性最常见恶性肿瘤之一，乳腺癌易发生隐匿性转移是乳腺癌患者的主要致死原因。课题组前期研究表明: TGF-β在乳腺癌细胞中可通过PI3K/Akt通路诱导Pokemon的表达，在乳腺癌细胞中Pokemon对E-cadherin的表达存在负性调控作用，而乳腺癌淋巴结转移与Pokemon的高表达呈正相关。目前Pokemon在乳腺癌转移过程中的作用及机制尚不明确。本课题拟揭示Pokemon 对乳腺癌细胞侵袭转移能力和乳腺癌上皮间质化的影响，阐明Pokemon促进乳腺癌发生侵袭转移的作用机制，分析在乳腺癌中Pokemon、E-cadherin 和Fibronectin 等表达的相关性，并探讨其是否与乳腺癌发生、转移和预后相关。本课题的完成将能明确TGF-β-PI3K/Akt -Pokemon - E-cadherin通路在乳腺癌上皮间质转化和侵袭转移中的作用，可为乳腺癌防治提供新的思路。

Breast cancer is one of the most malignant tumors in female , and the early tumor cell invasion and metastasis is the main cause for the death of breast cancer patients. Our previous work showed that:TGF-β could induce the expression of Pokemon via PI3K/Akt pathway in MCF-7 cells; Pokémon could negatively regulate the expression of E-cadherin in MCF-7 cells; the high expression of Pokemon was positively associated with lymph node metastasis .The role of Pokemon in breast cancer metastasis remains to be elucidated . This project will focus on: the effect of Pokemon on breast cancer invasion and metastasis ,the role of Pokemon in breast tumor epithelial mesenchymal transition, the associated mechanisms of Pokemon induced breast cancer metastasis, the correlation of Pokemon, E-cadherin and Vimentin expression in breast cancer and its association with the initiation, metastasis and prognosis of breast cancer. The completion of this project would uncover the role of TGF-β-PI3K/ Akt-Pokemon- E-cadherin pathway in breast cancer epithelial mesenchymal transition (EMT) and metastasis,and provide novel diagnostic and therapeutic approaches for breast cancer patients.