肝纤维化发病率高，目前无逆转肝纤维化的理想方法。骨髓间充质干细胞(BMSCs)是一种具有多潜能分化的细胞，在一定条件下可分化为肝样细胞，从而逆转和延缓肝纤维化，但BMSCs的临床疗效决定其归巢与分化,而SDF-1/CXCR4轴是影响归巢的关键信号通路。理论上,鳖甲煎丸一方面通过“活血、行血、祛瘀”保证BMSCs在血液中运行通畅，有利于BMSCs归巢，另一方面通过“养肝阴、补气血”改善肝脏微环境，保障BMSCs分化、再生营养。能否利用鳖甲煎丸抗肝纤维化的优势及多靶点作用的特性促进BMSCs抗肝纤维化，目前尚无研究。本项目以此为切入点，通过鳖甲煎丸诱导分化实验及干预BMSCs抗肝纤维化的实验，观察BMSCs移植后肝系细胞特异标志物（CK-18、ALB、AFP）及信号通路SDF-1/CXCR4等的变化，从BMSCs归巢及分化方面探讨鳖甲煎丸对干细胞治疗肝纤维化的影响，为中药联合干细胞治疗提供依据

Liver fibrosis is a high incidence of the disease,But there is no ideal way to reverse it . So it is an urgently need to find new treatments . The bone marrow mesenchymal stem cells (BMSCs) is a kind of differentiation of pluripotent cells, under certain conditions, can differentiate into hepatocyte-like cells, thereby reversing liver fibrosis . However, its clinical efficacy depend on BMSCs homing and differentiation , SDF-1/CXCR4 axis is a key signaling pathways on BMSCs homing.Theoretically , on one hand Biejiajianwan made the " blooding, Blooding Circulation, stasising" ensure BMSCs the smooth running in the blood , help BMSCs homing , on the other hand depending the " nourishing yin qi and nutring " on improving liver microenvironment protect BMSCs differentiation , regeneration nutrition. Can use Biejiajianwan strengths and multi-target anti- hepatic fibrosis characteristic of stem cells liver fibrosis? Nobody has studied. The project as a starting point, through Biejiajianwan intervention BMSCs induced differentiation experiments and experimental liver fibrosis , liver -specific cell lines (CK-18, ALB, AFP) was observed after BMSCs transplantation and SDF- 1/CXCR4 and other changes in terms of homing and differentiation of BMSCs investigate the effect of liver fibrosis Biejiajianwan treatment of stem cells. in a word ,it sprovide the basis for tem cells combined traditional Chinese medicine .