3D打印多孔钛合金种植体结合rhBMP-2/PDGF-BB壳聚糖微球涂层促骨结合作用的研究

如何提高种植体表面骨结合的强度以及缩短骨结合时间是目前的研究重点。研究发现三维复合多孔形貌的种植体表面比传统种植体更有利于骨结合形成，而3D打印技术能制造出具有精确表面多孔结构的种植体。rhBMP-2是具有明确的骨诱导活性的蛋白，PDGF-BB是最强效的生物成骨细胞，对软组织的愈合也有重要作用，目前有研究将rhBMP-2和PDGF-BB应用于多孔支架骨再生，却鲜有将rhBMP-2和PDGF-BB联合应用于3D打印多孔钛合金种植体骨结合研究。本研究将利用3D打印技术制造出与传统种植体外形一致的精确表面多孔结构的钛合金种植体，将rhBMP-2和PDGF-BB生物涂层制备于该类种植体表面，分析对比其与传统种植体的生物相容性、对前成骨细胞的黏附、增殖及分化的影响和成骨方面的差异，研究其成骨机制，为该钛合金种植体的后续研究如成骨机制研究等提供实验基础，并为并为后期该种植体应用临床提供理论基础。

It is importance to improve the strength of implant osseointegration and shorten the time of osseointegration in current researches .At present, compared with traditional implant, some researches showed that the three dimensional porous surface morphology of implant was more favorable for the osseointegration. Then, 3D printing technology has provided a new method for the production of implants with accurate surface porous structure . Generally, rhBMP-2 is known as a protein that has well-defined one inducing activity and has the ability to induce bone formation. PDGF-BB is the most potent biological osteoblast and plays an important role in the healing of soft tissue.P. Nowadays,many studies have focused on combined with rhBMP-2 and PDGF-BB to promost bone regeneration on porous scaffold, however, rarely combined with rhBMP-2 and PDGF-BB to enhance osseointegration on the 3D printed porous titanium alloy impants . Hence, this study will utilize the 3D printing technology to manufacture the titanium alloy implants , which with accurate porous surface structure and its body shape consistents with traditional implants. Then prepare the rhBMP-2 and.PDGF-BB as biological slow-released coating on the 3D printed titanium alloy porous implant surface . To analysis the biocompatibility, the influence of preosteoblast in adhesion, proliferation and differentiation, and osteogenesis of 3D printed/rhBMP-2/ PDGF-BB porous titanium alloy implants compared with traditional implants and study the mechnism of osteoblast. It will provide the experimental basis for the follow-up study of the mechanism on bone-formation about this 3D printed titanium alloy porous implant which coated with rhBMP-2 alone or combined with.PDGF-BB chitosan microspheres, and will provide theoretical data for the later use in the clinic.