基于腺相关病毒载体环路示踪技术探索皮层神经元调控排便反射的神经回路机制研究

排便是由高级中枢参与的神经调控行为，皮层中存在与排便有关的神经元，自发性排便调控的神经环路机制不明确。本课题组前期证实排便的低级中枢位于脊髓腰骶L1至S1节段，且与脑干内侧的Barrington氏神经核团关系密切；功能性脑成像暗示排便受到皮层活动调控，解剖学追踪实验表明桥脑接收来自皮层区域的投射，然后投射到桥脑的导水管灰质；据此我们推测：粪便从储存到排出的转换神经机制可能涉及桥脑之上的皮质回路，调控排便活动的神经反射客观存在两级系统，皮层特定神经元活动与排便反射行为密切相关，激活皮层特定神经元对脑桥功能区的投射引发排便行为。本项目采用基于腺相关病毒载体的环路示踪技术、光遗传学、化学遗传、在体光纤记录等技术，定位排便行为密切相关的大脑皮层特定神经元，确定皮层神经元通过投射脑桥触发排便行为，明确皮层神经元通过脑干功能区调控排便反射的神经回路机制，为今后治疗排便功能障碍相关疾病提供了新的思路。

Defecation is a neuro regulatory behavior involving the senior center. There are neurons related to defecation in the cortex, and the neural loop mechanism of spontaneous defecation regulation is not clear. Our team has previously confirmed that the lower center of defecation is located in the L1-S1 segment of the lumbosacral spinal cord, which is closely related to the Barrington's nucleus on the medial side of the brain stem; functional brain imaging suggests that defecation is regulated by cortical activity, and anatomical tracking experiments show that the pontine receives projections from the cortical region, and then projects to the aqueduct gray of the pontine; therefore, we speculate that: feces from storage to storage There are two levels of neuroreflexes regulating defecation activity. The specific cortical neuron activity is closely related to defecation reflexes. The specific cortical neuron can activate the projection of the specific cortical neuron to the pontine function area to trigger defecation behavior. In this project, loop tracking technology based on adeno-associated virus vector, photogenetics, chemical genetics, in vivo optical fiber recording and other technologies are used to locate specific neurons in the cerebral cortex closely related to defecation behavior, determine the neural loop mechanism of cortical neurons regulating defecation reflex through brainstem function area, and determine the future treatment Defecation dysfunction related diseases provide new ideas.