高寒泥炭湿地储存了大量的碳且对气候变化敏感。驱动碳转化的微生物和碳的稳定性之间有复杂联动关系，两者的耦联作用及对增温的响应机制尚不清楚。本项目基于高寒泥炭湿地的野外增温控制实验，分析湿地地上和地下生物量碳对增温的敏感性差异，测定土壤CO2、CH4净交换通量，靶定16SrRNA和28SrRNA基因、产甲烷功能基因mcrA和甲烷氧化pmoA功能基因，量化功能微生物群落结构和丰度，阐明其对土壤碳释放的决定作用。利用不依赖于底物的同位素18O-H2O标记新方法，量化土壤微生物碳利用效率和周转时间，阐明增温对参与调控有机质分解的微生物碳代谢过程的影响；分析土壤有机碳化学结构的特征和真菌与细菌的群落组成对增温的响应，探究土壤分解菌群落演变与有土壤有机质分解动态之间的耦联关系，揭示土壤碳稳定性的微生物调控机制。该研究对于探明区域碳平衡及其对气候变暖的反馈具有重要意义。

Alpine wetland stores a large amount of carbon and is sensitive to climate change. There is a complex linkage between microorganisms driving carbon transformation and carbon stability, and the coupling effect and response mechanism to warming are still unclear. In this project, an alpine peatland ecosystem on the Qinghai-Tibetan Plateau will be selected as a case to conduct a seasonal asymmetric warming experiment. First, we will analyze the sensitivity of aboveground and underground biomass carbon to warming, and measure the net exchange fluxes of CO2 and CH4 in soil. Targeting the 16S rRNA and 28S rRNA genes, mcrA and pmoA functional genes for methane production, the structure and abundance of microbial functional communities will be quantified and their decisive role in soil carbon release will be clarified. Second, a new method of isotope 18O-H2O labeling independent of substrate will be used to quantify soil microbial carbon use efficiency and turnover time, and to elucidate the effect of warming on microbial metabolism involved in the regulation of organic matter decomposition. In order to analyze the characteristics of soil organic carbon chemical structure and the response of fungi and bacterial community composition to warming, the coupling relationship between soil decomposition bacteria community evolution and organic C stability will be explored to reveal the microbial regulation mechanism of soil carbon stability. This will help to explore the regional carbon balance and its feedback on climate warming.