缓释小分子药物BIO透明质酸凝胶修复软骨缺损的研究

关节软骨缺损是常见病、多发病。我国软骨损伤患者达3000万以上。治疗软骨损伤性疾病有巨大的社会和经济效益。然而，目前由于在生物支架材料、种子细胞来源、细胞分化因子、构建组织工程软骨的形式等还存在各种不足，仍然无法得到天然软骨，课题组研究使用小分子化合物治疗软骨损伤性疾病，取得了一定的研究成果（Arthritis Research ＆therapy,2015；ACS nano, 2016）。本项目将使用缓释小分子有机化合物BIO水凝胶支架修复损伤的关节软骨。采用紫外点光源照射的方式软骨受损处成胶，通过吸引自身成体干细胞归巢来进行软骨修复。并实验阐明软骨修复过程中外源性细胞使用必要性以及系统阐明软骨修复过程中新生组织分化、发育及退变全过程。课题组目标（1）完善BIO缓释水凝胶系统，为软骨损伤难题找到一种安全、有效、临床可操作的治疗方式，（2）提出软骨修复新理论。

Cartilage defect occurs often. There are more than 30 million cartilage defect patients in China. There is huge social and ecomical benefits to treat these patients. However, lots of shortcomings are still present in the biomaterials, seeder cells, stimulating factors, and the way to construct engineered cartilage. So, it is still no way to get hayline cartilage. Our team have used small molecule to treat cartilage defect and have got some better results (Arthritis Research ＆therapy,2015；ACS nano, 2016). This project will use photo-cross-linked hydrogel with sustained small molecule BIO to restore cartilage injury. The project will use UV light to form the hydrogel in the cartilage defect. The mesenchymal stem cells will be recruited to the hydrogel and be used to form repair tissue. The small molecule BIO in the hydrogel will induce the repair tissue into cartilage. We will further study if it is necessary to introduce the exogenous cells and will study the differentiation, development and degeneration process of the repair tissue. Through this study, the project will find a useful and safe way to treat cartilage injury. And, new theory will be formed towards cartialge injury repair.