基于UPLC-QTOF技术和体内过程的合欢皮木脂素类物质抗焦虑活性物质基础研究

合欢皮是传统的具有镇静安神功效的中药，现有研究发现合欢皮的粗提取物具有抗焦虑活性，但对合欢皮抗焦虑活性成分缺乏系统的研究，尤其是其抗焦虑的活性成分及作用机制和作用靶点，目前尚未明了。本申请项目旨在解决这些关键问题。本课题组发现合欢皮木脂素类成分具有很强的抗焦虑活性。据此，本项目拟采用药理学、分子生物学、药物分析学、中药化学、药物化学等多学科交叉方法，应用UPLC-QTOF技术和构建国内外认可药理筛选模型,将体内过程与体外活性研究有机结合,系统地分离鉴定合欢皮抗焦虑的活性成分，从药物对焦虑症应激反应的神经-内分泌-免疫网络调控角度，在整体水平、细胞水平及分子水平探讨活性成分的作用机制，明确其作用靶点。以筛选的活性成分作为先导化合物进行结构修饰，研究构效关系，为抗焦虑药物的研发提供目标化合物，为合欢皮临床疗效提供科学依据，为开发抗焦虑的创新药物奠定基础。

Cortex Albiziae is one of the traditional Chinese herbs with tranquilizing effect.Many studies have recently shown that crude extract of Cortex Albiziae has significant antianxiety effect. But at present the material base of the antianxiety effect of Cortex Albiziae has not yet been confirmed, especially its active ingredients and the mechanism of action and the targets of drug action have not been elucidated. This application project aims to solve these key problems.Our research group has found that Cortex Albiziae lignanoids material has a significant antianxiety effect. Accordingly, Our research group plans to achieve an objective to optimize the best active ingredients and modify the chemical constitution of active ingredients through a method based on the UPLC-QTOF technology coupled with the study of the process in vivo and of setting up the pharmacological screening model proposed by experts at home and aboard. This is an application of interdisciplinary research techniques including pharmacology, pharmaceutical analysis science, molecular biology and chemistry of Traditional Chinese Medicine.It provides the organic combination of the study of the process in vivo and active material research in vitro.Meanwhile, the possible mechanism of antianxiety effect of active ingredients of the antianxiety effect of Cortex Albiziae lignanoids material is to be probed from the animal, cell and molecule levels from the aspect of nervous-endocrine-immune network.We believe that this study will contribute to offer target compounds in antianxiety drug development and find the acting targets, which will put forward to achieve integral study on therapeutic basis matter and action mechanism of Cortex Albiziae, and then to provide the scientific basis for the antianxiety clinical efficacy of Cortex Albiziae, lay the foundation for the development of new antianxiety drug.