中枢化学感受性调节在呼吸活动的稳态中起了重要的作用。在脑内多个区域可以检测到化学感受性反应，但是其机制仍不清楚。已知细胞外液pH下降能直接激活多种离子通道的门控特性，但是哪些离子通道实际上是质子门控的尚不清楚。我们的预实验结果显示TASK-1和ASIC1a在延髓多处与呼吸相关的核团上有共表达，因此TASK-1和ASIC1a可能共同参与H+浓度对呼吸的调节。本实验拟通过免疫组化、免疫荧光、电生理和药理学等方法（1）确定中枢化学感受器的定位及其细胞特性；（2）阐明TASKs和ASICs协同调节中枢化学感受性的机制。本研究的科学意义不仅能促进对中枢化学感受器的感受机制的认识，也对研究呼吸中枢调控障碍导致的相关疾病提供了新的思路。

Central chemosensitive regulation plays an important role in respiratory homeostasis. Chemosensitive responses have been detected in several brain areas, yet their precise mechanisms remain unclear. One established mechanism for chemosensitivity is the direct activation of ion channels by a reduced extracellular pH. Many ion channels are modulated by extracellular proton, however, it is still unknown which ion channels are actually proton gated. Our preliminary studies show that TASK-1 and ASIC1a were co-expressed in many respiration-related nuclei of medulla, so TASK1 and ASIC1a may coordinate respiratory responses during H+ changes in the lateral ventricle. In this project, immunohistochemical, immunofluorescent, electrophysiological and pharmacological methods will be used to (1) identify central chemoreceptors' location and their cellular behavior, (2) explore interactory mechanisms of TASKs and ASICs in contribution to central chemosensitivity. Significance of this study not only lies in a better understanding of sensing mechanisms of the central chemoreceptor, but also provides new strategies for searching pathological mechanisms about diseases of respiratory center dysregulation.