TGF-β1、OPN基因多态性与河南汉族类风湿关节炎肺间质病变证候演变的关系研究

类风湿关节炎肺间质病变是类风湿关节炎肺部受累主要表现形式，文献报道发病率约为11%，预后较差，严重威胁着患者健康，目前对该病研究较少。本项目通过提出“证候多样性-基因多态性”假说，采用聚类分析、因子分析、逻辑回归分析方法对380例河南汉族类风湿关节炎肺间质病变患者临床资料进行研究，采用聚合酶链式反应-限制性片段长度多态性方法测定患者及健康对照组的TGF-β1、OPN基因多态性，以阐明该病人群的中医证侯分布规律，分析其基因型和等位基因频率的分布，初步确定该病的易感人群，同时对其不同证候与TGF-β1、OPN基因多态性之间的内在关联及交互作用进行分析，从而揭示两者之间的关系。本研究将从分子生物学水平的一个侧面阐明该病的遗传发病机制，以及对证候演变规律的影响，为证候辨证寻找客观的物质基础，也为以后类风湿关节炎肺间质病变的基因诊断、基因治疗和进一步提高中医的诊治水平提供遗传学依据。

Rheumatoid arthritis-Interstitial lung disease(RA-ILD) is the main complications of rheumatoid arthritis, which accounted for 11% of total RA patients. The prognosis of the disease is very poor and it easily leads to a serious threat to the health of patients. So far,the study about the syndrome characteristics of the disease in TCM haven’t been conducted.According to the studies at present, we assume that there is a association between TCM syndromes and the genes’polymorphims in patients with RA-ILD in Henan. In order to confirm the view, We will collect the informations of 380 patients with RA-ILD in Henan and explore the regularity of the distribution of the TCM syndromes by the cluster analysis methed, factor analysis and the multivariate logistic regression analysis method. At the same time, we will explore the association between TGF-β1、OPN genes’polymorphisms and the disease and the TCM syndromes by the method of PCR-RFLP. The study helps to clarify the genetic pathogenesis of the disease and the disease syndrome characteristics. It will provide the genetic basis for the diagnosis, the treatment of TCM for RA-ILD in the future.