磁性分子印记聚合物SERS基底的构筑及对抗生素残留的检测

本项目拟将表面增强拉曼散射（SERS）技术和磁性分子印迹技术相结合对食品中残留的痕量抗生素进行检测。创新性地针对待测抗生素分子"量身定制"构筑SERS基底，并结合磁性分离技术，研究了分子印迹聚合物与SERS基底不同的复合方式对SERS增强机理的影响。本项目的重点在于如何将分子印迹聚合物引入到传统的SERS基底材料，达到二者的完美结合，分别设计了三种复合方式：将已合成好的印迹聚合物组装到制备好的银纳米粒子上；将制备好的银作为反应物置于分子印迹聚合物的制备过程中；将制备好的印迹聚合物置于银纳米粒子的合成过程中。通过比较，选择最优的SERS基底，不仅可以利用SERS本身的高灵敏度以及高选择性等，还兼顾了印迹聚合物的特异性、选择性、可重复利用等优势对食品中的抗生素残留进行简便、快速、高效、现场地检测，有利于发现和解决食品中抗生素残留问题的途径，具有重要的社会价值并为食品安全监督提供技术支撑。

The project intends to detect trace antibiotic residues in food by combined surface-enhanced Raman scattering (SERS) and magnetic molecular imprinting technique. It is an innovative research to "tailor-made" synthesize SERS substrate based on the antibiotic molecule to be tested combined with magnetic separation technology, which has studied different SERS ehanced mechanism effected by various combinition modes. This topic focuses on how to introduce molecular imprinted polymer (MIP) to the SERS substrate to form a perfect combination, which is designed for three manners: the synthesised MIP assembly to the prepared silver substrate; the prepared silver as a reactant added to the preparation process of MIP; the prepared MIP as a reactant added into the synthetic process of silver nanoparticles. The optimal SERS substrate is selected by compared ehanced effect from different prepared methods, which not only can take advantage of high sensitivity and selectivity of SERS itself, but also can take into account the specificity, selective and reusable of MIP. It may become a simple, rapid and efficient detection method for antibiotic residues in food. As a novel SERS substrate, it is useful to provide technical support for food safety supervision.