胃癌细胞伪足形成和延伸及细胞粘附是影响胃癌细胞迁移和侵袭的主要原因。Mena在调控细胞伪足形成和延伸、细胞运动过程中起着重要作用。我们前期研究表明，Mena在多数胃癌组织中高表达，其表达与淋巴结转移、肿瘤浸润深度显著相关，Mena高表达组胃癌患者预后较差；高表达Mena显著增强胃癌细胞的迁移和侵袭能力；Mena与细胞伪足蛋白Lpd、细胞粘附分子 TES都具有相互作用，且TES与Mena的结合抑制Mena与Lpd的结合。根据前期研究结果，我们推测Mena与Lpd/TES相互作用调节胃癌细胞伪足形成和延伸、细胞运动，进而影响胃癌细胞迁移和侵袭。本项目拟在前期研究基础上，深入探讨Mena与Lpd/TES相互作用对胃癌细胞伪足和细胞运动能力的影响，阐明Mena在胃癌细胞迁移和侵袭过程中的作用及分子机制。本研究对于阐明胃癌转移的分子机制具有重要意义，为胃癌转移患者分子靶向治疗提供新的治疗靶点和策略。

Formation and protrusion of cell pseudopodia and cell adhension play important roles in gastric cancer migration and invasion. Mena, a protein of Ena/VASP family, participates the regulation of pseudopodia formation and extension and cell motility. In previous studies, we found high Mena protein expression in most gastric cancer tissues compared with the matched adjacent non-tumor tissues. High Mena expression was significantly correlated with lymph node metastasis, deeper tumor infiltration, advanced TNM stages, and poor survival in gastric cancer patients. Ectogenic overexpression of Mena significantly enhanced the migration and invasion of gastric cancer cells. Knocking down expression of Mena significantly inhibited the migration and invasion of gastric cancer cells. Immunoprecipitation revealed that Mena interacted with Lpd (a protein localizes in pseudopodia regulating elongation of actin filaments) and TES (a cell adhesion protein regulating cell spreading). Furthermore, TES interacted with Mena, which inhibited the interaction between Mena and Lpd. Our research suggested that Mena may interact with Lpd/TES to regulate cell pseudopodia formation and extension and cell motility in gastric cancer, which influence the tumour cell migration and invasion. In our present study, the role of interaction between Mena and Lpd/TES in regulating gastric cancer cell migration and invasion is further investigated. This study can establish good fundation for understanding the molecular mechanism of cell pseudopodia in tumor metastasis, and suggest a potential target for the molecular targeting treatment of patients with gastric cancer metastasis.