膝关节骨性关节炎(Knee Osteoarthritis)是人类最常见的退变性骨病，已成为全球公共卫生关注的热点。软骨及软骨下骨是膝关节的基本结构和功能单位。研究表明OPG／RANKL／RANK系统在调节软骨及软骨下骨代谢方面具有重要作用，将可能成为骨关节炎治疗的靶点。.根据“肾主骨”和“经脉痹阻”等中医病机理论，以去双前肢诱导直立大鼠膝骨性关节炎模型和携带荧光素酶的报导基因单克隆细胞株为研究对象，采用细胞和分子生物学等方法，研究补肾通络中药介导OPG／RANKL／RANK系统调控软骨及软骨下骨代谢的机制，进一步奠定“补肾通络法”及其方药治疗骨退变性疾病的基础，深入探讨“久立伤骨损于肾及经脉痹阻”病机理论的现代生物学特性，揭示“肾主骨及经脉痹阻”中医病机理论的内在规律，建立“补肾通络法”理论现代生物学研究思路和方法，丰富和发展中医“肾主骨、经脉痹阻”和“补肾通络法”理论的科学内涵。

Knee osteoarthritis is the most common degenerative bone disease in humans, and also become a hot spot in global public health. Cartilage and subchondral bone are the basic structural and functional units of the knee joint. Studies have shown that the OPG/RANKL/RANK system plays an important role in the regulation of cartilage and subchondral bone metabolism. It may be a target for the treatment of osteoarthritis. .According to the theory of traditional Chinese medicine, such as "kidney bone" and "meridians of obstructions", setting up Rat model of knee osteoarthritis induced by double forelimbs and a monoclonal cell line carrying luciferase reporter gene as study subjects, Using methods such as cell and molecular biology. To study the mechanism of Bushen Tongluo traditional Chinese medicine mediating OPG/RANKL/RANK system on the regulation of cartilage and subchondral bone metabolism. To further lay the foundation for the treatment of degenerative bone disease with "Bushen Tongluo" and its prescriptions. In order to make the In-depth exploration of the modern biological characteristics of the pathogenesis theory of "long-term injury, injury to kidney and meridians, and obstruction of the kidneys", revealing the Internal Law of TCM Pathogenesis Theory of "Kidney Bone and Meridian Obstruction", establishing the Theory and Method of Modern Biology Research on the Theory of Bushen Tongluo, enriching and developing the scientific connotation of the theories of “kidney bone, Meridian Obstruction” and “Kidney Clearance Method”.