子宫内膜容受性（ER）异常是限制体外受精-胚胎移植（IVF-ET）技术妊娠成功率的主要因素。胶原纤维（CF）作为内膜细胞外基质（ECM）的重要组成，种植窗（WOI）期CF的动力学时空改变是影响ECM重塑和ER建立的重要环节，但CF合成和降解的调控机制尚未阐明。我们前期研究发现，常规控制性超促排卵（COH）过程中，超生理水平的激素环境可刺激WOI期内膜胶原过度增生从而影响ER。本课题拟对胶原进行分型和定量，并对胶原调节系统-uPA/PAI-1、MMPs/TIMPs和TGF-β在WOI期内膜的时序表达进行研究，并重点采用子宫内膜基质细胞的体外培养模型，观察和分析与着床密切相关的激素、蛋白酶、细胞因子及药物对内膜CF合成降解的调节机制，及其对ER的影响，为阐明胚胎着床失败机理和改进相关防治措施提供理论依据。

Abnormal endometrial receptivity (ER) is a major factor limiting the pregnancy success rate of in vitro fertilization - embryo transfer (IVF-ET) technology. As an important component of the endometrial extracellular matrix (ECM), the dynamic spatio-temporal change of collagen fibril (CF) during the window of implantation (WOI) period is an important part of the ECM remodeling and ER building. However, the regulatory mechanism of CF synthesis and degradation has not been elucidated. Our previous study found that the supraphysiological levels of hormones environment produced from conventional controlled ovarian hyperstimulation (COH) can stimulate endometrial collagen hyperplasia in WOI, and thus affecting ER. Based on this, we are proposed to further classify and quantify the collagen, to study the temporal expression of collagen conditioning system - urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), the matrix metalloproteinase (MMPs) and their inhibitors (TIMPs) and transforming growth factor (TGF-β) in WOI phase endometrium. And focus on endometrial stromal cells cultured in vitro model, to observe and analysis the regulation mechanism of above implantation closely related hormones, protease, cytokines and drugs on CF synthesis and degradation, and their affection on ER, so that to provide a theoretical basis for clarifing the mechanism of embryo implantation failure and improving related prevention and treatment.