PO2、PCO2和pH多功能荧光毛细管传感器构建及在实时血气分析监测中的应用

动脉血气实时监测分析在临床严重疾病诊断和及时治疗方案调整等方面具有十分重要的价值，本项目针对传统光纤实时监测传感器因光学构造及探针固定技术影响而以单参数监测为主，存在实时多功能并行监测方面的严重局限，探索将CO2、pH与O2荧光指示剂固定于单一毛细管中，开展高灵敏、多功能荧光毛细管传感器研究，解决PO2、PCO2和pH同波长荧光敏感分子发现与差异化多参数并行监测等两个关键科学问题，突破探针构建、光源和探测器及待测液协同调控与配置、多对象血气分析监测、一致性数据处理和系统再优化等关键技术，并进一步开展人体血液和家兔体内PO2、PCO2和pH试验检测。在此基础上，围绕实时临床应用场景，开展监测数据和临床疾病数据对比研究和分析，完成系统有效性评估，建立评价方法。项目将针对性地发展实时血气分析多功能传感器基础理论，为多生物参数并行联合检测的临床及其有效性应用奠定技术基础。

Real-time monitoring and analysis of blood gas is of great significance in disease diagnosis and treatment protocols adjustment. At present, the conventional optical fiber sensors mainly focus on single parameters owing to their own optical structure and probe fixation technology, and have great limitations in real-time multifunctional monitoring of blood gas. This proposed work intends to design a PO2, PCO2 and pH multifunctional fluorescent capillary based sensor. The CO2, pH and O2 fluorescent indicators with the same excitation wavelength are fixed in a single capillary tube respectively, in order to realized multi-parameter monitoring. By optimizing the configuration of light source, detector and liquid control system, the capillary sensor with high sensitivity and multi-parameter detection can be prepared. The sensor is used to detect PO2、PCO2 and pH in human blood and rabbits, and real-time analysis of blood gas can be realized. On this basis, we further study the real-time clinical application by analyzing the monitoring data and clinical data to obtain the multi-factor combined treatment and its effect evaluation. In summary, this proposed research will develop basic theory of multifunctional sensor for real-time blood gas analysis, and support the clinical application of multi-parameter biological detection.