基于DNA折纸逻辑门的动物性食品痕量激素类污染物阵列式荧光传感快速检测技术研究

近年来，动物性食品中多种激素类物质的滥用不仅严重危害人体健康，而且造成了极大经济损失，因此，日益受到全世界高度关注。本课题针对激素类痕量复合污染物快速检测技术缺乏的现状，以典型激素类污染物（己烯雌酚、雌二醇、孕酮等）为目标物，采用DNA折纸技术制备可寻址自组装的核酸适配体纳米探针，结合功能微球和多孔聚合物运用到动物性复杂食品基质前处理，实现目标物的高特异性分离富集；其次，融合DNA折纸和DNA分子逻辑门的技术，提出“DNA折纸逻辑门”概念，阐明其信号调控机制，以功能化纳米组装复合物为载体建立DNA折纸逻辑门的阵列式多通道荧光传感技术，实现动物性食品中激素类复合污染物的痕量快速精准筛查。本课题是食品卫生学、纳米生物技术和生物传感技术等多学科交叉前沿性研究，其研究成果可用于食品安全、检验检疫、环境监测等领域，对推动食品中有害物质痕量、精准快速检测新技术发展具有很好的理论价值和应用。

In recent years, the abuse of various hormonal substances in animal foods has not only seriously endangered human health, but also caused great economic losses. Therefore, it has been receiving more and more attention all over the world. This study selects typical hormone pollutants (diethylstilbestrol, estradiol, progesterone, etc.) as targets, in view of the lack of novel rapid detecting technologies for trace complex hormones pollutants at present. Based on DNA origami technology, the aptamer probes can be developed addressable self-assembling, which can be combined with functional microspheres and porous polymers for the pretreatment of complex animal food substrate, thus achieving the goal of highly specific separation and enrichment of targets. Next, we integrate the technologies of DNA origami and DNA molecular logic gates, put forward the concept of "DNA origami logic gate", and clarify its signal transduction mechanism. More importantly, the array type multichannel sensing platform can be set up based on DNA origami logic gate and the functional nano-complex assembly, hoping to realize the rapid and accurate screening of hormone compound pollutants in animal food. This subject is a challenging, pioneering and interdisciplinary research topic, involving food health, nanobiotechnology and other subjects. The results of this research can be applied in the fields of food health safety, entry-exit inspection and quarantine, environmental monitoring and so on. It is of great theoretical value and application prospects for promoting the development of new technologies, achieving rapid detection on trace amounts of food harmful pollutants.