紫杉醇诱导三维培养的iPS-NSCs-TrkC细胞分化修复脊髓损伤

脊髓损伤（spinal cord injury，SCI）再生修复是世界性难题。优化局部微环境、移植细胞均是修复SCI的重要手段。本项目拟建立稳定表达神经营养因子NT3受体TrkC基因的iPS细胞；构建NT3修饰的神经再生支架材料（LOCS-CBD-NT3）用于三维培养稳定表达TrkC基因的由iPS诱导的神经干细胞（iPS-NSCs-TrkC细胞），研究LOCS-CBD-NT3三维培养体系对iPS-NSCs-TrkC细胞的功能影响和作用机制；探讨紫杉醇诱导三维培养的iPS-NSCs-TrkC细胞向限制性神经元前体细胞的分化、机制及用于大鼠急性全横断脊髓损伤修复的可行性。期望通过LOCS提供“土壤”，iPS-NSCs-TrkC细胞作为“种子”，NT3提供“营养”，构建具有较高生物活性的神经网络组织，探索其脊髓损伤修复作用。本项目具有明显的创新性，有望为SCI修复提供新思路。

Regenerative repair of spinal cord injury (SCI) is a worldwide challenge. Both remodeling local microenvironment and transplanting cells to the injured area are important approaches in SCI repairing research. In this project, we will generate NSCs induced by iPS cells stably expressing TrkC, which is named as iPS-NSCs-TrkC cells, and construct NT3-modified LOCS that is named by LOCS-CBD-NT3, a biomaterial tailored for neuroregeneration, for the three-dimensional culture. We will also study its characteristics and their underlying mechanisms of iPS-NSCs-TrkC cells, as well as the differentiation features and underlying mechanisms of iPS-NSCs-TrkC cells into neuronal restricted precursors induced by Taxol in three-dimensional culture. We will also investigate the repairing efficacy of those cells for rat with acute spinal cord transectional injury. Briefly, aiming to construct the regenerative neural network tissue by using LOCS as the "soil", iPS-NSCs-TrkC cells as the "seeds", and NT3 as the "nutrient", this project is obviously innovative and may provide a new approach for SCI repairing.