最新研究表明，介导免疫耐受的调节性T细胞（Treg）与介导炎症反应的Th17细胞都来源于初始T细胞，两者的功能和分化过程相互对抗、转化，类似于中医阴阳对立统一关系，其平衡失调影响着类风湿关节炎（RA）的发生与转归。 前期研究表明，温阳散寒湿除法对RA具有较好临床疗效，显著抑制胶原诱导性小鼠关节炎，并能促进Treg免疫细胞分泌免疫抑制性细胞因子IL-10。基于此，本课题拟以Th17细胞/ Treg分化可塑性为切入点，利用DBA/1小鼠胶原关节炎、iTreg/Th17体外诱导分化和转化细胞模型，从T细胞的扩增、细胞因子的分泌、特异转录因子的表达、分化和相关转化等层面，深入研究温阳散寒除湿法调控Th17/Treg细胞平衡而介导治疗类风湿关节炎的作用机理，并以此为切入点解析"温阳散湿除湿法治疗痹证（寒湿痹阻）"科学内涵，为临床遣药组方、提高疗效和新药研究奠定理论基础。

Recently, the balance of regulatory T cell(Treg) and type 17 helper 17 cell (Th17) was broadly reported to be critical to maintain the immunotolerance of the immune system, while the brokendown of this balance will resulted in the development of autoimmune disease. Treg cells has potent immunosuppressive function. However, recent reports showed that Treg cels could not suppress everything, and even that Treg cells could be polarized into pro-inflammatory Th17 cells in the autoimmune disease. Thus,the reciprocal suppression and transition between Treg and Th17 cells really make the pathogenesis of autoimmune disease complicated, and also represent the typical Ying and Yang theory in the traditional chinese medicine. Previous studies have shown that WenYangSanHanChushi method has a dramatic clinical efficacy in treating rheumatoid arthritis (RA),significantly inhibited collagen (CII)-induced arthritis (CIA) in mice and promoted Treg cells immune suppressive cytokine IL-10 production. In this study, we will focus on the the Developmental Plasticity of Th17 and Treg cells to elucidate the mechanisms of WenYangSanHanChuShi method to treat RA.Briefly,through investigating the amplification of T cell, cytokine secretion, the expression of specific transcription factor in the animal model of collagen-induced arthriti in DBA/1 mice,in vitro differentiation of Th17 and Treg cells and the Treg cells converting into Th17 cells，in depth to study the WenYangSanHanChushi method regulates Th17/Treg cell balance mediated mechanism for treatment of RA. From this pointcut, we resolve the scientific connotation of "WenYangSanHanChushi method treats Bi Syndrome (HanShiBiZu Syndrome)",lay a theoretical foundation to improve the clinical efficacy of the treatment with RA, drug research and QianYaoZuFang.