MicroRNA介导的ProDH/脯氨酸通路变化对偏关苜蓿抗旱性的正调控作用研究

苜蓿被业界誉为“牧草之王”。在众多制约苜蓿规模种植和产业升级的因素中，干旱是最主要的胁迫因子之一。偏关苜蓿是分布于中国黄土高原地区的一种优良地方种质。本课题组以偏关苜蓿脯氨酸降解关键酶——脯氨酸脱氢酶的生物学通路作为研究靶点，利用网站WMD3设计针对控制偏关苜蓿ProDH的microRNA。以Ubi作为表达启动子，将其整合进表达载体pSB130中，构建pSB-UbimiR载体转化偏关苜蓿，对microRNA、靶向基因及蛋白的表达水平、耐盐力、抗旱性进行综合评价，以检测鉴定转基因操作效果。本项目的实施，将为利用转microRNA提高苜蓿抗旱性提供一定的理论和方法学基础。

Medicago sativa L. is always recognized as the queen of forage. Drought is one of the most prominent factors that restrict the distribution and production of Medicago sativa L. in a large scale. Medicago sativa cv Pianguan is an excellent native germplasm distributed in loess plateau of China. The research group focuses on the biological pathway of proline dehydrogenase (ProDH), which is the key enzyme in proline degradation. The microRNA that targets the ProDH gene will be designed by WMD3 (Web MicroRNA Designer 3) and integrated into the expression vector pSB130 with the expression promoterUbi, finally transforming pSB-UbimiRvector into Medicago sativa cv. Pianguan. From this study, the expression level of the microRNA, target gene and protein would be detected to evaluate the salt resistance and drought tolerance of Medicago sativa cv. Pianguan. It may also provide theoretical and methodological basis for improving the drought tolerance of alfalfa.