包虫病是由细粒棘球绦虫的蚴虫寄生人或家畜所引起的人畜共患病，因包虫病的病程较长，感染机制复杂，宿主在受到病原体感染后，通过自身免疫系统具有部分的抵御能力，但由于病原体与宿主间经长期进化形成了免疫逃逸的能力，使得感染后会产生不同的后果，有的人成为患者，有的人不发病。这为包虫病的诊断及后续的治疗带来了困难。本研究首先计划开展对细粒棘球绦的成虫、虫卵、六钓蚴、原头蚴四个发育阶段的总mRNA进行测序，利用生物信息学的技术确定各阶段的表达谱并进行基因注释。然后对各阶段的表达谱进行差异分析，寻找出各发育阶段具有特异性的分子，并分析其生物学功能。希望据此为包虫病的发育、免疫相互作用等研究积累资料。在此基础上分别开展人及家畜特异性诊断抗原分子的筛选研究，希望能筛出特异性和灵敏性强的，具有实际临床应用价值的诊断抗原分子，为人及家畜包虫病的早期诊断和应用打下基础。

Hydatid disease is a zoonotic disease caused by the larval stage of Echinococcus granulosus. After infection by the pathogen, some hosts become resistant to the pathogen and thus are disease-free through activation of their immune system; while others become patients because the pathogen developed in such ways to acquire the ability to escape from hosts' immunity during prolonged evolution process. It is the long disease duration and the complex infection mechanisms that make diagnosis and treatment for hydatid disease difficult and inefficient. We firstly propose to sequence the mRNA of adult worm, eggs, oncosphere and protoscolex to determine gene expression patterns in different development stages using bioinformatics methods, identifying distinctly expressed genes for individual developmental stages in order to provide the data for development and host-parasite immune interaction of Echinococcus granulosus. Our goals are to identify molecular targets that can determine specific developmental stages of Echinococcus granulosus and develop novel methods to quickly and accurately diagnose hydatid disease for humans and livestock.