稀土铈调控蒙古韭老化种子细胞程序性死亡（PCD）的信号机制研究

种子老化是自然界的普遍现象，也是种质资源保存中面临的一个严重问题，且老化中的细胞程序性死亡（PCD）研究是当前植物学和生理生化研究的热点问题之一。本项目融合了特色材料—蒙古韭和特色资源—稀土铈，以短命老化种子的PCD信号转导为结合点，以贮藏3年、5年和7年的未处理的蒙古韭（有活力CKⅠ、无活力CKⅡ）种子为对照，采用新的实验设备和方法（如电感耦合等离子体质谱法、激光共聚焦显微镜定位法和半定量PCR法）从细胞、亚细胞及分子水平将深入研究稀土铈、老化和稀土铈+老化处理后的种子细胞程序化死亡的Ca-CaM和ROS中的类Caspase-3信号转导及相关基因表达；同时将稀土铈在老化种子细胞中累积部位的超微定位表征结果与PCD特征进行关联分析，为揭示稀土铈在老化种子PCD过程中的信号机制提供依据，同时为延长种子寿命、提高活力、资源保存及稀土铈农用奠定基础。

Seed aging, a common phenomenon in nature, is also a serious problem in the preservation of Germplasm Resources. The programmed cell death in aging is one of the hot topics in plant genetics, physiology and Biochemistry. This project combines the specialty materials of Allium mongolicum Regel and specialty resources of rare earth, The PCD signal transduction of short-lived seed was used as the binding site, the Allium mongolicum Regel seed stored for 3 years, 5years and 7 years as CK, The Ca-CaM in PCD and Caspase-3 signal transduction of ROS and the expression of related genes of the seeds treated by Ce3+, aging and Ce3+ and aging were studied. At the same time, the relationship between the location of the Ce3+ in the aging seed cells and the PCD characteristics were analyzed. The results of the study will provide a basis for revealing the mechanism of Ce3+ in the process of aging seed PCD, and to lay the foundation for prolonging the longevity of seeds, improving the vitality, saving resources and the application of rare earth.