猪初始态胚胎干细胞（ESCs）的建系及其诱导分化为功能性配子技术体系的建立，不仅可以为人类不孕不育等疾病的治疗提供良好的动物模型，还可以为未来猪干细胞育种体系的建立提供理论依据。本项目基于本实验室前期研究基础，拟开展猪ESCs的建系及其定向分化为雄性生殖细胞的研究。分析和验证猪囊胚内细胞团特异表达基因，优化猪ESCs体外培养体系，建立猪ESCs系。检测和验证猪原始生殖细胞（PGCs）特异标记基因并构建其报告载体，筛选调控PGCs发育的关键因子，建立猪ESCs定向分化为雄性生殖细胞的技术体系。对诱导不同阶段的生殖细胞进行生殖细胞特异基因和蛋白的检测、表观遗传因子的检测以及减数分裂为功能性精子等发育潜能的检测，以期获得有功能的精子，并建立猪ESCs定向分化为雄性生殖细胞的技术标准，阐明其调控机制，为未来在临床医学和家畜育种中的相关应用提供理论依据和技术支持。

The establishment of technical systems of naïve porcine embryonic stem cells (ESCs) derivation and functional gametes induction from ESCs could not only provide good animal models for treatment of human diseases such as infertility, but also provide the theory basis for the pig stem cell breeding systems establishment. Based on the previous research in our lab, the project plans to derive porcine ESCs and then directionally differentiate them into male germ cells. First, we will analyze and confirm specific expression genes in porcine blastocyst inner cell masses, and generate porcine ESC lines through optimization of porcine ESCs culture systems in vitro. Second, we will analyze and confirm porcine primordial germ cells (PGCs) specific genes, construct the reporter systems and then screen key regulation factors of PGCs development. The technical systems of induction of male germ cells from porcine ESCs could be thus established and optimized. Finally, we will detect the developmental potential of the induced germ cells in different stages, including the detection of germ cell marker genes and proteins expression, the epigenetic detection and the functional gametes generation after meiosis. We wish to get functional gametes and establish the technical standards of induction of male germ cells from porcine ESCs, which could provide theory basis and technology supports for the application of clinical medicine and livestock breeding in the future.