尽管冠脉血运重建治疗使许多冠心病患者获益，但仍存在血管再狭窄、费用高等不足。目前的研究重点，集中在改善内皮祖细胞数量和功能以促进血管新生方面，但尚无成熟的临床药物可供使用。许多研究证明益气养阴活血法可促进血管新生并改善内皮祖细胞的数量和功能，但缺乏对相关成熟方剂的深入研究。养心通脉方具有益气养阴，活血通脉之功，临床疗效可靠。据此，我们分离培养内皮祖细胞，观察养心通脉方含药血清对其增殖、迁移和粘附能力的影响。其次运用静脉给药和缺血心肌局部给药的不同方式，观察含药血清预处理内皮祖细胞对急性心肌梗死兔的梗死面积的影响，并同步测定缺血心肌区和主动脉粥样硬化斑块内的微血管密度及血管生长因子水平，阐述益气养阴活血法对内皮祖细胞数量和功能的影响，并观察该法预处理内皮祖细胞的促血管新生作用及副反应的发生情况，为益气养阴活血法促缺血心肌血管新生提供科学依据，并为进一步寻找更合理的内皮祖细胞移植方法提供借鉴。

Although many Coronary Heart Disease patients benefitted from revascularization of coronary, but the blood vessel may restenosis and the cost was high.The experts tried to solve it by improving the quantity and function of endothelial progenitor cells(EPCs), but the effective medicine was still absent. Many studies showed that replenishing Qi, nourishing Yin and activating Blood Circulation could improve the quantity and function of EPCs and promote angiogenesis, but they were still not thorough enough. Yangxin Tongmai Recipe could replenishing Qi, nourishing Yin and activating Blood Circulation. According to the former studies, we try to separate EPCs and observe the influence of endothelial progenitor cell pretreatmented with Yangxin Tongmai Recipe on proliferation, migration and conglutination of EPCs. Then we observe the influence of EPCs pretreatmented with Yangxin Tongmai Recipe on infarction size. We measure the microvessel density and the level of expression of angiogenesis factors proteins and DNA in the periphery zone and in atherosclerosis plaques in rabbit model with acute myocardial infarction. Base on the results above, we try to expound the effect of replenishing Qi, nourishing Yin and activating Blood Circulation on the quantity and function of EPCs, while looking for the scientific evidence for neovascularization in ischemic myocardium by the method and the more reasonable method for transplantation of EPCs.