肠粘膜屏障功能失调是粘连性肠梗阻（AIO）形成的重要因素，肠道菌群可维持肠粘膜屏障结构稳定，AhR通路参与肠粘膜屏障功能的调控。课题组前期工作证实以大黄-桃仁为药对的诸多方剂在外科临床治疗AIO临床疗效显著，并结合网络药理学筛选出其效应的核心靶点网络，为此提出科学假说：大黄-桃仁药对防治AIO的机制可能与调控AhR信号通路影响Th17/Treg稳态重塑肠道菌群作用肠粘膜屏障有关。本研究拟①以Caco-2细胞为研究对象，采用siRNA干扰和过表达观察大黄-桃仁对LPS刺激Caco-2细胞活性及AhR信号通路的影响及其作用途径；②以AIO大鼠为研究对象，采用16s rRNA测序、RT-PCR、WB等分子生物技术，从肠道菌群变化、AhR通路相关指标表达方面探讨大黄-桃仁干预AhR通路介导肠粘膜固有层Th17/Treg平衡重塑肠道菌群修复粘膜从而实现其防治AIO的作用的具体机制。

Intestinal mucosal barrier is the trigger point of the formation of adhesive intestinal obstruction (AIO). Intestinal flora plays an important role in maintaining the stability of function of intestinal mucosal barrier. AhR pathway is involved in the synthesis and secretion of intestinal mucosal barrier. The previous work of our research group confirmed that many prescriptions with herb pairs such as Rhei Radix Et Rhizoma and Persicae Semen (RRER-PS) were clinically effective in the treatment of AIO. Based on the network pharmacology, the core target network of the RRER-PS treated AIO was screened. Accordingly, we made a hypothesis: the mechanism of RRER-PS in the treatment of AIO may be related to remodeling intestinal flora to affect intestinal mucosal barrier and the regulation of AhR signaling pathway. In this study, Caco-2 cells were used as the research objects to study the effect of RRER-PS on Caco-2 cell activity and adhesion protein, as well as the pathway by applying small interfering RNA and overexpression, cell culture and molecular biology techniques. In addition, with AIO rats as the research objects, hematoxylin-eosin staining, 16s rRNA sequencing analysis, immunohistochemistry, Western blotting were applied to discuss the expression of AHR pathway related index, confirm the molecular mechanism of RRER-PS in the prevention and treatment of AIO to mediate intestinal mucosa Th17/Treg balance remodeling intestinal flora.