尘螨特异性免疫治疗可有效治疗特应性皮炎（AD），但由于疗程长和不良反应常导致脱落率较高，目前迫切需要研发不良反应小和免疫效能高的尘螨疫苗。纳米疫苗是综合解决抗原、佐剂和靶向性的良好方案。我们在前期研究中构建了数种Langerin介导的靶向朗格汉斯细胞（LCs）的尘螨纳米疫苗，发现可有效被人单核细胞来源LCs（MoLCs）内吞，活化后的MoLCs可诱导初始T细胞向Th1和调节T细胞分化。.为探索尘螨纳米疫苗诱导免疫耐受防治AD的效果和机制，本研究拟构建PLGA-Derp1/Derf1-CpG-Langerin疫苗，以MoLCs、人组织皮片和尘螨致敏AD小鼠为研究对象，分析疫苗活化LCs和指导皮肤和外周血T淋巴细胞极化的作用，研究疫苗对尘螨致敏AD小鼠的预防和治疗作用，分析疫苗诱导免疫耐受的机制。可望较全面地解决尘螨纳米疫苗治疗AD的可行性和有效性，阐明其机制。经查新，国内外未见相关研究报道。

House dust mites(HDM) is the major allergen of patients with atopic dermatitis(AD). Evidence based analysis have proved the efficacy of HDM-specific immune therapy(SIT) for AD. Because of the treatment length and systemic side-effects, the drop-out rate is higher. Development of less adverse effects and higher immune potency of HDM vaccine is urgent needed. Nanopartical vaccine is the best integrated resolution for antigen, adjuvant and targeting therapy. We have constructed several nanoparticles of HDM targeting LCs through Langerin, and found it could be efficaciously endocytosed by monocyte derived LCs(MoLCs), activated MoLCs can induce naïve T cells polarization to Th1 and Treg. In order to clarify the immune tolerance mechanisms induced by HDM-PLGA vaccine targeting Langerhans cells through Langerin for the prevention and treatment of atopic dermatitis, we are intended to construct PLGA-Derp1/Derf1-CpG-Langerin vaccine, and use human MoLCs, human skin and HDM sensitized AD mouse model as research models to explore: ①Effects of HDM nanopartical vaccine on LCs activation and residential skin T cells and naïve T cells polarization; ② Therapeutical and preventive effects of HDM nanopartical vaccine on HDM-sensitized AD mouse model, ③Mechanisms of epicutaneous immune tolerance induced by HDM nanopartical vaccine. We are expected to completely figure out the feasibility and effectiveness of HDM nanopartical vaccine in treatment of AD, and clarify the immune tolerance mechanisms of our designed vaccine. After novelty search, we could not find any relative research reports so far.