在前期研究中，申请者鉴定出一个新的水稻突变体hfa-1,其叶色白化转绿、多分蘖矮杆性状受隐性核基因hw-1(t)控制。综合前人研究结果，初步推断hw-1(t)为在单子叶植物中首次发现的新型叶色突变基因，其通过影响类胡萝卜素合成产生白化和多分蘖矮杆表型。为证实上述假设，本项目从生理、分子和蛋白等方面开展下列研究：（1）hfa-1内源激素（细胞分裂素、独角金内酯）和类胡萝卜素合成中间化合物测定；（2）在明确hw-1(t)基因功能的基础上，利用荧光定量和基因芯片技术分析hw-1(t)和类胡萝卜素生物合成途径相关基因的互作关系；（3）利用酵母双杂技术，筛选与Hw-1(t)相互作用的表达蛋白。通过上述研究阐明hw-1(t)调控类胡萝卜素合成途径的分子机理，进而探讨hw-1(t)在水稻光合作用、形态构建等发育网络中的重要作用。研究结果对深入了解类胡萝卜素生物合成在单子叶植物中的调控机制具有重要意义。

In the preliminary studies, we identified a novel rice mutant named hfa-1, whose green-revertible albino, dwarf and high tillering were controlled by a recessive gene hw-1(t). By summarizing prior researches, it was indicated that hw-1(t), which affected the biosynthesis of carotenoid to generate an albino, dwarf and high tillering phenotype, was the first leaf-color mutant gene in monocotyledons. To verify the above assumptions, this project would from the physiological, molecular and protein levels to carry out the following researches: (1) determinate the biosynthesis intermediates of endogenous hormones (cytokinins, strigolactones) and carotenoid in hfa-1; (2) based on clear function of hw-1(t), Fluorescence Quota PCR and Gene Array will be used to detect the interaction between hw-1(t) and genes involved in carotenoid biosynthesis pathway; (3) with the yeast two-hybrid system, expressed proteins that interacted with hw-1(t) would be screened. Through above-mentioned studies, the molecular mechanism of hw-1(t) regulate carotenoid biosynthesis pathway will be clarified, and the important role of hw-1(t) in rice photosynthesis and morphosis may be investigated. The results of this project are of great significance to reveal the regulation mechanism of carotenoid biosynthesis in monocotyledonous.