MAAs-蛋白质-氧化多糖抗紫外、可修复复合膜构建及其机制

以蛋白质、多糖等为原料的复合膜具有安全、环保等特点，但机械性差、活性物质的迁移释放不可控一直是其瓶颈问题。解决的关键是要提高材料间的相容性，改善结合方式。申请人团队在研究中发现类菌孢素氨基酸（MAAs）具有良好的紫外阻隔性、抗氧化性和抑菌性，可通过成肽反应与蛋白质形成共聚物。而蛋白质和氧化淀粉则可通过Schiff base reation动态共价交联。本项目将在研究MAAs、氧化多糖和蛋白质结构及功能特性的基础上，重点围绕三者的分子自组装、成肽反应和Schiff base reaction诱导的多重交联过程展开，构建MAAs-蛋白质-氧化多糖的松散耦合系统。通过优化基质浓度及配比、pH、温度等因素，改善复合膜特性，构建出具有良好紫外阻隔、抑菌、自愈合能力的MAAs-蛋白质-氧化多糖活性复合纳米纤维膜。同时研究静电共价耦合下的分子构象转变，阐明各分子间的互作规律对膜性能的影响机制。

Composite film based on polysaccharide, proteins are characterized with safety, environment-friendly et al, while the poor mechanical property and uncontrollable migration and release of active substances have limited its wide utilization. Previous reports have pointed that the key process is to improve the compatibility of material and enhance the bonding force between active material and wall material. According to our previous research, MAAs have good UV barrier, anti-oxidation and antibacterial properties. Furthermore they could combine with proteins by peptides reaction. The products of Schiff base reaction between proteins and oxidized polysaccharide exhibited excellent self-healing, antibacterial, mechanical properties et al. In this project, the structure and functional properties of MAAs, oxidized polysaccharides and proteins would be analyzed firstly. Then we will emphasize on the multiple cross-linking process induced by molecular self-assembly, peptide reaction and Schiff base reaction to construct MAAs-protein-oxidized starch loosely coupled system. By adjusting the matrix concentration and ratio, pH, temperature and other factors, the properties of the composite membrane would be improved, and a MAAs-protein-oxidized polysaccharide active composite nanofiber membrane with good UV blocking, antibacterial, and self-healing capabilities could be constructed. Meanwhile, we will clarify the mechanism of molecular conformational transformation during the process as well as the interaction between the molecular on membrane performance. Finally, we could obtain the mechanism of macromolecule interaction and its effect on the membrane performance.