慢性中耳炎是在耳鼻咽喉科领域中较常见的疾病，鼓膜穿孔是其常见的并发症，慢性鼓膜穿孔需要手术（鼓膜修补术）治疗，但手术费用昂贵，具有风险，故需要在门诊治疗的慢性鼓膜穿孔修补的方法，然而至今还没有。本项目研究要点包括构建动物的慢性鼓膜穿孔模型，探讨干细胞在鼓膜上的分布特征，通过实验分析鼓膜上皮干细胞增殖与分化规律，利用生长因子促进鼓膜穿孔的修补，本研究的门诊治疗的技术，替代传统的鼓膜修补手术，在提高我国慢性鼓膜穿孔修复的治疗水平具有重要意义。

Chronic otitis media is one of the most common diseases in the otolaryngological department, and tympanic membrane (TM) perforation is a common complication. Most chronic TM perforations require surgical interventions such as tympanoplasty. However, the surgical procedures have disadvantages, for instance, high cost, anesthesia-associated risks. There are no effective methods in outpatient department for treating chronic TM perforations yet. In the present study, chronic TM perforation models will be established, to investigate stem cell distribution in TM, through analyzing epithelial stem cell proliferation and differentiation, to promote the chronic TM perforation repair by using growth factor. In the present study, the technology for chronic TM perforation repair in outpatient department will be an alternative to traditional surgery; will be significant in improving treatment of chronic TM perforation in our country.