男性不育症是临床常见病，弱精子症是导致男性不育症的主要原因之一。弱精子症的最新研究表明，精子DNA甲基化异常与弱精子症的发生密切相关；结合课题组前期临床观察，发现补肾生精活血中药芪芎方治疗弱精子症安全有效，能够提高精子活动力。从而，本课题提出假说：精子DNA甲基化异常是引起弱精子症从而导致男性不育的原因之一，芪芎方是通过干预DNA甲基化信号通道治疗弱精子症。课题采用目前较公认的弱精子症大鼠模型，给予芪芎方干预后，观察大鼠精子活动力，以亚硫酸氢盐克隆测序法检测精子印记基因CpG岛甲基化，结合染色、光镜观察睾丸、附睾组织结构变化，从病理结构和DNA表达水平揭示芪芎方治疗弱精子症的可能作用机制，为补肾生精活血法治疗弱精子症提供进一步的理论基础，为临床男性不育症的治疗提供参考。

Male infertility is a common disease in andrology patients, and asthenospermia is one of the main causes of male infertility. Latest research showed that DNA methylation was closely related to occurrence of asthenospermia. In the clinical practice，our research team found that Qi Xiong Fang was effective on asthenospermia significantly compared with sham group, which can improve sperm motility safely. With these precondition, now the hypothesis is proposed: sperm DNA methylation disorder is one of the main causes of asthenospermia,which induced.infertility, and Qi Xiong Fang treat asthenospermia by regulating sperm DNA methylation. A generally recognized rat model is employed, then the model rats cerceive administration of Qi Xiong Fang. Samples of sperm are prepared and the vitality are tested respectively. Sperm DNA methylation is determined by BSP, combined with staining and pathological tests on structure change of testis and epididymis. This proposal aims at exploring mechanism of Qi Xiong Fang for asthenospermia both from morphology and DNA expression, and will lay a.pharmacological foundation for "nourishing Kindney, generating Essence and activating Blood"Chinese medicine on asthenospermia treatment.