建立P35转染的粘虫新细胞系及其病毒离体复制的研究

用P35抗细胞凋亡基因转染已建立的粘虫细胞系，筛选抗凋亡、高表达的细胞株，克隆高毒Φ牟《局辏芯扛貌《纠胩甯粗频奶匦裕秆「弑泶锏南赴?-高毒力的毒株组合，为病毒拇罅可脱芯坎≡镉爰闹鞯南嗷ス叵堤峁┮谰荩兄匾睦砺垡庖搴褪导τ眉壑怠

Insect Cell lines which display various characteristics have been widel y used for studies of insect pathogens, specially insect viruses, and for recombinant protein production. However, it is not possible to predict the potential usefulness and biological characteristics of new cell lines from primary culture cell source. Genetic modification of insect cell line is now technically feasible by stably transfacting insect cells. Introduction of specific genes of know functions into insect cells could potentially achieve significant improvement of the cells towards desired characteristics.the baculovirus encoded protein P35 is an inhibitor of apoptosis of insect cells triggered by baculovirus infection. In this paper we report a stable transformation of insect cells, NNEAU Ms7311, with P35 gene which led to a significant improvement of the cell lines in their growth characteristics,production of virus, susceptibility to virus, stress in PBS,and level of recombinant protein production.