拟禾本科根结线虫（Meloidogyne graminicola）是一种为害隐蔽、繁殖速率高、致病力强的植物寄生线虫，是诱发水稻根结线虫病的主要病原之一。近年来，该线虫在我国南方水稻产区快速蔓延，严重威胁水稻安全生产。FMRF酰胺类多肽（flp）基因介导的神经信号传递对线虫的运动、取食和寄生起着重要作用。本研究以线虫基因组学为基础，运用比较功能基因组分析手段筛选flp同源基因，结合RT-PCR和RACE方法，获得拟禾本科根结线虫flp基因的cDNA全长序列，利用原位杂交的方法对flp基因进行定位，深入分析其功能；通过体外合成dsRNA，对拟禾本科根结线虫二龄幼虫进行离体干扰试验，同时，采用QPCR方法分析病原线虫mRNA表达量；选择RNAi效果良好的flp基因进行多基因RNAi效应验证。拟禾本科根结线虫flp基因的获得及其功能的解析，可为水稻根结线虫病防控提供新的理论依据。

Meloidogyne graminicola was a kind of harm concealment, high reproduction rate, strong parasitic and, was one of the main pathogen of rice root knot nematode diseases. In recent years, M. graminicola spread rapidly on rice in south China and, was threated to rice production. FMRFamide-related peptides (flp) gene mediated neural signal transmission, it plays an important role for nematodes with feeding, moving and parasiting. This study based on the nematode genomics, comparison function genome analysis means to screen flp gene homologous genes.The full length cDNA sequence of flp gene was obtained by RT-PCR and RACE from M. graminicola. Flp gene was localized by in stiu hybridization and, analysis of its function. Through the in vitro synthesis of dsRNA and , using M. graminicola J2 to carry out inierference effects in vitro validation . At the same time, using QPCR method to analysis of mRNA. Choose the best effect of RNAi flp genes for much of RNAi effect validation. Obtain M. graminicola flp genes and analysis their functions can provide new theoretical basis for rice root knot nematode disease prevention and control .