中非五种猪SLA I递呈非洲猪瘟病毒多肽的分子机制研究

近年来，非洲猪瘟在我国大面积流行，给养猪业造成了巨大的经济损失，国家急需对其病原致病性和宿主抗感染免疫开展相关研究以保障养殖业健康发展。研究表明，ASFV能感染几乎所有品种的中国家猪和地方野猪，但是却不感染一些非洲地方野猪或感染后宿主不会表现出明显的临床症状，即不同地方猪种对ASFV的抗性不同。鉴于主要组织相容性复合体 I（MHC I）与疾病抗性相关，同时也是抗病毒CTL免疫应答中的核心分子，我们选定了两种非洲地方野猪（疣猪、假面野猪）、两种中国地方野猪（西藏香猪、黑山野猪）和一种中国商品化家猪（大白猪），对其MHC I分子进行功能和结构研究：分析其对ASFV抗原多肽的结合规律和亲和力高低，鉴定筛选出ASFV 的CTL表位；解析不同猪种MHC I-多肽－β2m复合体的三级结构，从原子水平阐述其递呈ASFV抗原多肽的分子机制，为我国ASFV的CTL 表位疫苗研制和猪的抗病育种提供数据支撑。

In recent years, African swine fever (African swine fever, ASF) has become widespread in China, causing huge economic losses to the pig industry. Research on its pathogenicity and host anti-infection immunity is urgently needed to ensure the healthy development of the breeding industry. Studies have shown that ASFV can infect almost all breeds of Chinese domestic pigs and local wild boars, but does not infect some local wild boars in Africa, or just without clinical symptoms after infection, that is, different ASFV resistance exists in different local pig breeds. Since major histocompatibility complex class I (MHC I) is associated with disease resistance and is a core molecule in the immune response of antiviral cytotoxic T lymphocytes (CTL), we want to focus on MHC I molecules of two kinds of African wild boars (Phacochoerus africanus, Potamochoerus larvatus), two kinds of Chinese local wild boar (Tibetan pig, Heishan pig) and a Chinese commercial domestic pig: analyse their affinity with ASFV antigen peptides, identify and screen ASFV CTL epitopes, resolve tertiary structures of MHC I- peptide-β2m complex. The results may explain the molecular mechanisms of different MHC I in ASFV antigen presentation, and provide data for the development of CTL epitope vaccine of ASFV, as well as the pig disease-resistant breeding.