嫁接是提高蔬菜抗逆性的有效途径，ABA在调控植物对非生物胁迫的响应中发挥重要作用，然而ABA在嫁接诱导植物耐冷性中的作用机理尚不清楚。本项目针对日光温室低温逆境导致黄瓜代谢受阻，生长受抑，产量和品质降低等问题，拟以‘金妈妈5号（M5）’南瓜和‘津优35号’黄瓜为试材，互为砧穗嫁接，并用ABA和ROS及其抑制剂或清除剂进行处理，通过生理和分子生物学手段开展研究：1）分析低温下砧木和接穗ABA的动态变化及其与耐冷性的关系；2）探讨砧-穗中的ABA对低温的交互应答方式；3）揭示ABA与ROS的相互作用及其在嫁接提高黄瓜耐冷性中的信号途径。该研究可为深入开展ABA参与植物砧-穗互作及其信号转导途径研究提供借鉴，对筛选抗逆性强的砧穗组合，缓解日光温室黄瓜低温逆境障碍具有重要意义。

Grafting is an effective way to improve the stress tolerance of vegetable. ABA plays an important role in regulating plant to response to abiotic stress. However, no information has been provided on the mechanism of ABA involved in grafting-induced low temperature intensity tolerance of plants. This work focuses on the problems, such as the retardation of metabolism, limitation of growth, decrease in yield and quality of cucumber caused by low temperature stress in solar-greenhouse. In the study, the ‘Jinmama5(M5)’ pumpkin and ‘jinyou 35’ cucumber are used as the experimental materials and grafted with each other. Combining with the use of donor and inhibitor or scavenger of ABA and ROS, we will carry out the following researches by the means of physiological and molecular biology: 1) to analyze the dynamic change of ABA in rootstocks and scions as well as the relationship between ABA and chilling tolerance of plant under low temperature intensity;2) to explore the ways of ABA in rootstock and scion and the interaction response to low temperature;3) to reveal the cross and interaction of ABA and ROS, and the signal pathway of ABA in the chilling tolerance of cucumber enhanced by grafting. Our results can provide guidance for the further study of stock-scion communications and the signal transduction pathway of ABA and also are significant in selecting stock-scion combination with high stress tolerance and alleviating the low temperature adversity of cucumber in solar-greenhouse.