花生是豆科喜磷作物，对供磷水平反应敏感，有效磷匮缺是我国花生生产的重要限制因素。提高磷吸收效率是花生生产节本增效的重要途径。作物活化土壤无效磷的能力和吸收能力是决定磷吸收效率的两个关键因子。本项目以前期筛选的不同磷吸收效率花生基因型为试验材料，检测低磷胁迫处理后磷素吸收效率、根系分泌有机酸的种类和数量、酸性磷酸酶活性、磷转运蛋白基因表达量、蛋白质组成及变化规律变化等，比较基因型间磷活化能力和吸收能力的差异，明确限制磷吸收效率的因素是活化能力还是吸收能力，发掘调控花生磷素活化和吸收的关键基因及网络，解析花生对磷素高效吸收及耐低磷的分子机制。这为栽培上研制施肥或土壤调理技术、育种上开发磷高效分子标记、培育磷吸收效率高的品种提供理论基础，从栽培和育种两个途径实现资源高效利用，达到“双减”目标。

Peanut (Arachis hypogaea L.) is a phosphorus loving leguminous crop. It is very sensitive to phosphorus fertility. Available phosphorus deficiency is an important factor which limits the improvement of peanut yield in china. So increasing phosphorus uptake efficiency is an important way to increase yield of peanut. Crop phosphorus activation ability and absorption ability are key factors that determining phosphorus absorption efficiency, which are very different between genotypes. The project is to take a series of peanut genotypes with different phosphorus efficiency as test materials, which were screened out in our previous study. Under the stress of low phosphorus, the kinds and amount of organic acids, H+ concentration, acid phosphatase activity, protein composition and variation, and relative expression of Pi transporters genes are to be detected in root and its secretion. Furthermore, we will study the phosphorus activation ability and absorption ability differences between peanut genotypes and their relationship with phosphorus efficiency. Reveal the key root exudates affecting phosphorus activation and the key gene determining the phosphorus absorption capacity. And point out which is the limiting factors for phosphorus efficiency, phosphorus activation ability or absorption ability. Guided by the above theory, new technology aimed at regulating phosphorus activation or absorption efficiency can be developed, and also related technology of regulating soil phosphorus supply and fertilizer. Moreover, breeders can develop new varieties with high phosphorus efficiency based on molecular markers associated with high phosphorus efficiency. The goal of "double reduction" will be achieved from cultivation or breeding way.