EV71病毒是人手足口病的主要病原，严重威胁我国婴幼儿身体健康和生命安全。已有研究发现，nucleolin作为EV71病毒的受体分子，可以介导病毒入侵敏感细胞。但nucleolin介导EV71病毒入侵细胞的具体机制尚不清楚。本项目拟通过病毒标记和感染可视化方法，实时观察量子点标记的EV71病毒入侵表达nucleolin细胞系的全过程，重点研究nucleolin介导的EV71病毒跨膜转运过程、囊泡运输过程、脱衣壳释放核酸过程，同时结合经典生物学研究手段，解析nucleolin介导病毒侵染细胞的入侵途径。本项目的完成，将为深入理解EV71病毒侵染细胞机制和抗病毒研究提供新的理论基础。

Enterovirus serotype 71 （EV71）is the major causative agent of human Hand, Foot and Mouth Disease(hHFMD) which threatened the health of infants. As an important receptor of EV71, how nucleolin mediated the infection of EV71 remains to be elucidated. In this program, we will study the cell entry process and mechanism of EV71 through virus labeling and dynamic visualization tecthnology, combining with traditional biological strategies. We will focus on the trans-membrane transport, the vesicular transport and the uncoating and RNA release process of EV71 during nucleolin mediated infection. This program would help to understand the infection mechanism and pathogenesis of EV71, and provide useful information for anti-EV71 research.