高粱蜡不仅对高粱抗逆具有重要作用，而且在食品、医药和化工生产中应用广泛。迄今为止，关于高粱蜡质合成相关基因的克隆还未见报道。本实验室在高粱BTx623突变体库筛选过程中，发现了一个茎秆蜡层缺失突变体sb1 (sorghum bloomless 1)，该突变表型由隐性单基因控制。本研究拟利用扫描电镜、透射电镜和气相-质谱联用技术检测sb1突变体蜡质晶体结构、表皮超微结构和蜡质组分含量的变化；分析高粱蜡层缺失后离体叶片失水速率和叶绿素浸出率的变化；利用已构建好的BC1F2突变体基因定位群体，采用Super-BSA混池测序法定位和克隆突变基因sb1，转化基因验证功能，并结合基因表达谱分析，阐明SB1基因在高粱蜡质合成代谢中的作用及蜡质形成的分子机制。深入挖掘高粱蜡质相关基因，对探究高粱蜡形成的分子机制、蜡对抗旱性的影响及深入开发高粱蜡资源都具有非常重要的意义。

Sorghum wax not only plays a vital important role in sorghum resistance, but also is widely used in food, medicine and chemical production. Up to now, the wax synthesis gene of sorghum has not been cloned. The stem wax-layer deficient mutant sb1 (sorghum bloomless 1) was controlled by a single recessive nuclear gene which was isolated by screening the BTx623 mutant library in our lab. Our study want to detect the waxy crystal structure, the epidermis ultrastructure, the wax composition and content of sb1 mutant by using scanning electron microscopy, transmission electron microscopy and gas chromatography–mass spectrometry technologies. To detect the rates of chlorophyll leaching and water loss in the excised leaves of the stem wax-layer deficient mutant. Super-BSA mixed pool sequencing method is applied to map and clone sb1 mutant gene using the BC1F2 mapping population which have been built earlier. Genetic transformation will be used to verify the gene function. And according to the gene expression profile analysis, we will clarify the role of SB1 and the molecular mechanism of wax synthesis. Cloning and identification wax synthesis genes of sorghum are very important for revealing the molecular mechanism of sorghum wax formation, the role of sorghum wax for drought resistance and in-depth development of sorghum wax.