菌核病是由腐生真菌核盘菌所导致的一种世界性病害，是对十字花科、茄科、豆科等经济作物威胁较为严重的植物疾病之一。本项目拟设计并构建一个小分子探针系统，在体检测菌核病发生进程中包括液泡加工酶、Caspase-3-like及Metacaspases在内的多种植物重要凋亡蛋白酶的活性。并在此基础上鉴定出所感染细胞凋亡蛋白酶的激活差异。随后，综合多种抗性指标，分析相关蛋白酶在植物抵抗核盘菌入侵过程中发挥的作用。项目的开展对于探索能否通过改变这些凋亡蛋白酶活性来削弱菌核病的发生具有重要意义。本项目所发展的检测方法可为其他植物蛋白酶的在体研究提供新的技术借鉴。

Sclerotium rot is a worldwide plant disease caused by the necrotrophic fungal pathogen Sclerotinia sclerotiorum. It is also one of the serious disease threaten to economic crop species including Brassicaceae, Solanaceae, Leguminosae. In this work, we intend to in vivo detection of the important plant proteases activity in the process of Sclerotinia sclerotiorum invasion via a designed small molecule probe system. And on this basis, the difference for apoptotic protease activation in the Sclerotinia sclerotiorum-infected cells will be identified. Then, combining with some resistance parameters, we will analyze the role of related proteases in plant resistance to Sclerotinia sclerotiorum infection. The project is of great significance to explore whether weaken the Sclerotinia sclerotiorum invasion by manipulating the apoptotic protease activity. The platform developed in this project provide a technical reference for in vivo studying other plant proteases.