丙型肝炎树鼩模型的研究及普通级广西树鼩种群的建立

丙型肝炎病毒（HCV）感染是全球严重的公共健康问题之一，目前尚无有效的预防HCV感染的疫苗。缺乏合适的动物模型和细胞培养体系是制约丙型肝炎相关研究的瓶颈。当前的初步实验研究结果提示树鼩有可能是除人和黑猩猩以外能感染HCV的小动物品系。目前用于科研的树鼩主要来自云南,广西野生树鼩实验动物化及其种群的建立对我国实验动物资源保种和生物医学应用等方面具有重要的意义。本项目通过对广西野生树鼩的驯养、繁殖、育幼等方面开展研究，建立起普通级广西树鼩繁殖和驯养种群；在我们多年来从未间断进行的树鼩感染乙肝病毒模型工作的基础上，通过静脉、腹腔、皮下、脾脏等不同接种途径、利用不同的HCV亚型的人感染血清和HCV转染细胞等不同的感染源，开展广西和云南两种普通级的树鼩体内和体外感染丙肝病毒的研究，并建立一套适合于检测树鼩HCV感染标志物的灵敏和稳定的方案，以探讨建立可靠的树鼩丙型肝炎模型。

Hepatitis C virus (HCV) infection is a serious global public health problem. There is no effective preventive vaccine HCV infection now. Lack of appropriate animal models and cell culture system to limiting the related study of hepatitis C. Tree shrew may be an alternative animal model to human and chimpanzee in hepatitis C research. It is great significance of laboratory animal resources conservation and biomedical applications by establishment of experimental animal populations in Guangxi wild tree shrews. This study aims to establish the breeding and domesticated populations in Guangxi wild tree shrews by domesticated and breeding the wild tree shrews. To study the infection of hepatitis C virus in vivo and in vitro by ues two general class tree shrew of Guangxi and Yunnan, through intravenous injection, intraperitoneal injection, subcutaneous injection and spleen injection, and different subtypes of HCV infected serum and HCVtransfected cells. To establish a sensitive and stable solution of detecting the tree shrew HCV infection markers, and then explore the establishment of a reliable animal model of hepatitis C in tree shrews.