食管癌是常见的恶性肿瘤，手术切除是其重要的治疗手段，但中晚期患者术后转移或复发率高，化疗毒性反应大；放射治疗虽然适用广；但单纯放疗存在肿瘤的辐射抗性和大剂量射线对正常组织严重损伤的难题，因此需要寻求新的治疗方法； Egr-1基因属于调控作用的转录因子家族，可直接感受电离辐射等因素的刺激, 在γ射线照射下能强烈诱导下游基因表达；研究表明，很多恶性肿瘤和肿瘤旁组织中elF4E过度表达，与肿瘤血管生成及侵袭转移能力相关;本项目拟构建以elF4E基因为靶向的RNA干扰质粒与肿瘤放疗敏感性启动子调控的融合自杀基因yCDglyTK相结合的治疗体系,以无毒副作用的磷酸钙纳米为载体，将该基因治疗体系导入食管癌细胞中，达到增强基因治疗靶向性及放疗增敏性的目的。研究内容：研究纳米与基因治疗体系的结合比率及转染效率；探讨该体系体内外杀伤食管癌细胞和放射增敏作用及其机制；研究结果将为临床食管癌基因治疗提供新的思路

Esophageal is common malignant tumor, surgical resection is an important treatment method, but the postoperative recurrence or transfer middle-late high, toxic reaction chemotherapy; Radiation treatment although extensive application; But pure existence of radiotherapy cancer radiation resistance and large dose of radiation damage to normal tissues serious problem, so need to search for new treatment method; Egr-1 gene belongs to the regulation effects transcription factor family, can feel directly ionizing radiation of the factors such as exciting, in the gamma rays can illuminate strong induction downstream gene expression; Studies show that many malignant tumors and tumors in the organization elF4E excessive expression, and invasion and metastasis of tumor angiogenesis and related ability; This project is to construct the elF4E gene targeting of RNA interference plasmid and tumor radiation sensitive regulation of the promoter fusion yCDglyTK suicide gene combination treatment system, non-toxic side effects of nanometer calcium phosphate as the carrier, the gene therapy of esophageal cells into system, to enhance gene therapy target of radiotherapy sensitization and the purpose of sex. The research content: study the nanometer and gene therapy ZhuanRan ratio and the combination of the system efficiency; Discusses this system in vitro and in vivo or kill esophageal tumor cell function and mechanism. The results will be for clinical esophageal therapy provides for new ideas.